Reference Report

Reference
Citation	Christensen, S., Cook, K., Cook, K. (2008.7.11). Isolation and characterization of Df(3L)BSC561. (Export to RIS)
FlyBase ID	FBrf0205008
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(3L)BSC561 Date: Fri, 11 Jul 2008 13:22:29 -0400 (18:22 BST) Isolation and characterization of Df(3L)BSC561 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC561 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06298 and PBac{WH}Lasp[f06993]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; P{XP}d06298/PBac{WH}Lasp[f06993] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC561 from the segment of P{XP}d06298 to the left of its FRT site and the segment of PBac{WH}Lasp[f06993] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC561 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 73A2;73C1. Df(3L)BSC561 failed to complement argos[W11], st[sp] and Df(3L)Exel6130. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX
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Language of Publication	English
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Data from Reference
Aberrations (2)
	Df(3L)BSC561 Df(3L)Exel6130
Alleles (2)
	aos^W11 st^sp
Constructs (1)
	P+PBac{XP5.WH5}
Genes (2)
	aos st
Insertions (3)
	P{XP}aos^d06298 P+PBac{XP5.WH5}BSC561 PBac{WH}Lasp^f06993