Reference Report

Reference
Citation	Geng, C., MacDonald, P.M. (2007). Identification of genes that influence gurken expression. Fly 1(5): 259--267. (Export to RIS)
FlyBase ID	FBrf0205043
Publication Type	Research paper
PubMed ID	18836312
PubMed Abstract	Axial patterning in Drosophila relies on the deployment of patterning proteins at specific regions within the developing oocyte. This process involves transport of mRNAs from the nurse cells to the oocyte, localization of mRNAs within the oocyte, and translational regulation of these mRNAs to restrict the final distribution of the proteins. Despite extensive analysis, the events of deployment are not fully understood and it seems certain that many contributing factors remain to be identified. We describe the development and application of a sensitized genetic screen to reveal such additional factors. Overexpression of Imp, a factor implicated in regulation of gurken mRNA, causes a weak dorsalization that can be enhanced by reducing the level of other factors acting in the same pathway. A collection of deficiency mutants was screened using this assay, leading to the identification of 5 genes that are candidates to contribute to axial patterning. Three of the genes were characterized in greater detail. The mushroom body expressed gene was implicated in axial patterning, with overexpression leading to a range of patterning abnormalities that can be explained by a more primary defect in organization of the cytoskeleton. Two mitotic cell cycle control factors, cyclin E and E2f1, were also implicated, raising the possibility that a mitotic cell cycle checkpoint may impinge on grk expression, much as meiotic checkpoints can alter expression of this gene.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Fly
Title	Fly
Publication Year	2007-
ISBN/ISSN	1933-6934 1933-6942
Data from Reference
Aberrations (20)
	Df(2L)Exel7063 Df(2L)Exel7070 Df(2L)Exel8038 Df(2R)Exel6056 Df(2R)Exel6058 Df(2R)Exel7094 Df(2R)Exel7095 Df(3L)ED4978 Df(3L)Exel6137 Df(3R)Exel6162 Df(3R)Exel6164 Df(3R)Exel6165 Df(3R)Exel6186 Df(3R)Exel6191 Df(3R)Exel6203 Df(3R)Exel6274 Df(3R)Exel6280 Df(3R)Exel7313 Df(3R)Exel7316 Df(3R)Exel9012
Alleles (58)
	Aos1^c06048 Cdc16^EY12544 CG8712^EY07021 CG8713^e00867 CG11210^KG08546 CG15256^f04709 CG17083^d00403 CG17202^f01979 CG18477^KG07704 CG42750^f03296 cnc⁰³⁹²¹ Cul-4^EP2518 Cul-4^KG02900 CycE⁰⁵²⁰⁶ CycE^AR95 CycE^KG07848 desat1^BG00955 desat1^EY07679 Dip-C^f00706 E2f⁰⁷¹⁷² E2fⁱ² Fas3^E25 Imp^Scer\UAS.cGa jigr1^BG00794 Khc^{E97.Hsp83.T:Ecol\lacZ} l(2)SH0983^SH0983 l(2)SH1181^SH1181 l(2)SH1622^SH1622 l(2)SH1919^SH1919 lace² lace^k05305 lig^e04268 lig^KG08209 msi¹ msi² mub⁰⁴⁰⁹³ mub^BG00074 mub^Scer\UAS.cGa ncm^BG01636 ncm^SH0931 Ntf-2r^EY05573 Ntf-2r^KG00588 Or35a^f02057 orb^dec pnt² pnt⁰⁷⁸²⁵ Pp1-87B¹ Pp1-87B^j6E7 RpS26⁰⁴⁵⁵³ RpS26^KG00230 Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} slv^KG02506 sna¹⁸ sqd^ix50 sut1^d07339 sut3^KG10160 Tim17b2^KG07430 Vps28^k16503
Constructs (5)
	P{Khc^E97:lacZ} P{matα4-GAL-VP16} P{SUPor-P} P{UAS-Imp.G} P{UASp-mub}
Genes (79)
	Acp36DE Aos1 AP-2μ bcd bsf Cdc16 CG8712 CG8713 CG11210 CG12769 CG15256 CG17083 CG17202 CG18477 CG42750 cnc Cpn Cul-4 CycE Cyp4e1 Cyp4e2 Cyp6a13 Cyp6a14 Cyp9f2 Cyp313a2 Cyp313a3 Cyp313a5 desat1 desat2 Dip-C DNApol-α180 E2f Fas3 fzo grk Imp Irk1 jigr1 kek3 Khc l(2)SH0983 l(2)SH1181 l(2)SH1622 l(2)SH1919 lace lig Mal-A1 Mal-A2 Mal-A3 msi mub ncm Nop56 Nrx-1 Ntf-2r Or35a orb osk Pglym87 pnt Pp1-87B Prosα2 PyK Rpn7 RpS26 Sas Scer\GAL4 slv sna Spp sqd stau sut1 sut2 sut3 T-cp1 Tim17b2 tRNA:CR32449 Vps28
Insertions (32)
	P{EP}Cul-4^EP2518 P{EPgy2}Cdc16^EY12544 P{EPgy2}CG8712^EY07021 P{EPgy2}desat1^EY07679 P{GT1}desat1^BG00955 P{GT1}jigr1^BG00794 P{lacW}bsf^SH1181 P{lacW}l(2)SH0983^SH0983 P{lacW}l(2)SH1622^SH1622 P{lacW}l(2)SH1919^SH1919 P{lacW}Vps28^k16503 P{matα4-GAL-VP16}V2H P{matα4-GAL-VP16}V37 P{PZ}CycE⁰⁵²⁰⁶ P{SUPor-P}CG11210^KG08546 P{SUPor-P}CG18477^KG07704 P{SUPor-P}Cul-4^KG02900 P{SUPor-P}CycE^KG07848 P{SUPor-P}lig^KG08209 P{SUPor-P}Ntf-2r^KG00588 P{SUPor-P}slv^KG02506 P{SUPor-P}sut3^KG10160 P{SUPor-P}Tim17b2^KG07430 P{XP}CG17083^d00403 P{XP}sut1^d07339 PBac{PB}Aos1^c06048 PBac{RB}CG8713^e00867 PBac{RB}lig^e04268 PBac{WH}CG15256^f04709 PBac{WH}CG17202^f01979 PBac{WH}Dip-C^f00706 PBac{WH}Or35a^f02057
Natural transposons (1)
	P-element