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Citation
Geng, C., MacDonald, P.M. (2007). Identification of genes that influence gurken expression.  Fly 1(5): 259--267.
FlyBase ID
FBrf0205043
Publication Type
Research paper
Abstract
Axial patterning in Drosophila relies on the deployment of patterning proteins at specific regions within the developing oocyte. This process involves transport of mRNAs from the nurse cells to the oocyte, localization of mRNAs within the oocyte, and translational regulation of these mRNAs to restrict the final distribution of the proteins. Despite extensive analysis, the events of deployment are not fully understood and it seems certain that many contributing factors remain to be identified. We describe the development and application of a sensitized genetic screen to reveal such additional factors. Overexpression of Imp, a factor implicated in regulation of gurken mRNA, causes a weak dorsalization that can be enhanced by reducing the level of other factors acting in the same pathway. A collection of deficiency mutants was screened using this assay, leading to the identification of 5 genes that are candidates to contribute to axial patterning. Three of the genes were characterized in greater detail. The mushroom body expressed gene was implicated in axial patterning, with overexpression leading to a range of patterning abnormalities that can be explained by a more primary defect in organization of the cytoskeleton. Two mitotic cell cycle control factors, cyclin E and E2f1, were also implicated, raising the possibility that a mitotic cell cycle checkpoint may impinge on grk expression, much as meiotic checkpoints can alter expression of this gene.
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Fly
    Title
    Fly
    Publication Year
    2007-
    ISBN/ISSN
    1933-6934 1933-6942
    Data From Reference
    Aberrations (20)
    Alleles (57)
    Genes (78)
    Natural transposons (1)
    Insertions (31)
    Experimental Tools (2)
    Transgenic Constructs (5)