Open Close
Reference
Citation
Ghildiyal, M., Seitz, H., Horwich, M.D., Li, C., Du, T., Lee, S., Xu, J., Kittler, E.L., Zapp, M.L., Weng, Z., Zamore, P.D. (2008). Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells.  Science 320(5879): 1077--1081.
FlyBase ID
FBrf0205170
Publication Type
Research paper
Abstract

Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded RNA (dsRNA) as a defense against viral infection. We identified endogenous siRNAs (endo-siRNAs), 21 nucleotides in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo-siRNAs complementary to messenger RNAs (mRNAs); these siRNAs disproportionately mapped to the complementary regions of overlapping mRNAs predicted to form double-stranded RNA in vivo. Normal accumulation of somatic endo-siRNAs requires the siRNA-generating ribonuclease Dicer-2 and the RNAi effector protein Argonaute2 (Ago2). We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line.

PubMed ID
PubMed Central ID
PMC2953241 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Lai lab miRNA expression and conservation data for Drosophila.
Mohammed and Lai, 2016.2.8, Lai lab miRNA expression and conservation data for Drosophila. [FBrf0230987]

Note

Molecular biology. Slicing and dicing for small RNAs.
Birchler and Kavi, 2008, Science 320(5879): 1023--1024 [FBrf0215858]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075 1095-9203
    Data From Reference
    Alleles (3)
    Genes (6)
    Cell Lines (1)
    Natural transposons (26)
    Transgenic Constructs (1)