Reference Report

Reference
Citation	Benoit, P., Papin, C., Kwak, J.E., Wickens, M., Simonelig, M. (2008). PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila. Development 135(11): 1969--1979. (Export to RIS)
FlyBase ID	FBrf0205291
Publication Type	Research paper
PubMed ID	18434412
PubMed Abstract	Cytoplasmic polyadenylation has an essential role in activating maternal mRNA translation during early development. In vertebrates, the reaction requires CPEB, an RNA-binding protein and the poly(A) polymerase GLD-2. GLD-2-type poly(A) polymerases form a family clearly distinguishable from canonical poly(A) polymerases (PAPs). In Drosophila, canonical PAP is involved in cytoplasmic polyadenylation with Orb, the Drosophila CPEB, during mid-oogenesis. We show that the female germline GLD-2 is encoded by wispy. Wispy acts as a poly(A) polymerase in a tethering assay and in vivo for cytoplasmic polyadenylation of specific mRNA targets during late oogenesis and early embryogenesis. wispy function is required at the final stage of oogenesis for metaphase of meiosis I arrest and for progression beyond this stage. By contrast, canonical PAP acts with Orb for the earliest steps of oogenesis. Both Wispy and PAP interact with Orb genetically and physically in an ovarian complex. We conclude that two distinct poly(A) polymerases have a role in cytoplasmic polyadenylation in the female germline, each of them being specifically required for different steps of oogenesis.
DOI	10.1242/dev.021444
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(1)RA47
Alleles (7)
	hrg^PAP21 hrg^PAP45 orb^mel wisp^12-3147 wisp⁴⁰ wisp⁸⁹ wisp^KG05287
Constructs (1)
	P{?SUPor-P}
Genes (14)
	bcd BicC c(3)G cort CycA CycB Gld2 hrg nos orb osk RpS2 smg wisp
Insertions (3)
	P{?SUPor-P}wisp⁴⁰ P{?SUPor-P}wisp⁸⁹ P{SUPor-P}wisp^KG05287