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Citation
Marygold, S.J., Roote, J., Reuter, G., Lambertsson, A., Ashburner, M., Millburn, G.H., Harrison, P.M., Yu, Z., Kenmochi, N., Kaufman, T.C., Leevers, S.J., Cook, K.R. (2007). The ribosomal protein genes and Minute loci of Drosophila melanogaster.  Genome Biol. 8(10): R216.
FlyBase ID
FBrf0205398
Publication Type
Research paper
Abstract

Mutations in genes encoding ribosomal proteins (RPs) have been shown to cause an array of cellular and developmental defects in a variety of organisms. In Drosophila melanogaster, disruption of RP genes can result in the 'Minute' syndrome of dominant, haploinsufficient phenotypes, which include prolonged development, short and thin bristles, and poor fertility and viability. While more than 50 Minute loci have been defined genetically, only 15 have so far been characterized molecularly and shown to correspond to RP genes.We combined bioinformatic and genetic approaches to conduct a systematic analysis of the relationship between RP genes and Minute loci. First, we identified 88 genes encoding 79 different cytoplasmic RPs (CRPs) and 75 genes encoding distinct mitochondrial RPs (MRPs). Interestingly, nine CRP genes are present as duplicates and, while all appear to be functional, one member of each gene pair has relatively limited expression. Next, we defined 65 discrete Minute loci by genetic criteria. Of these, 64 correspond to, or very likely correspond to, CRP genes; the single non-CRP-encoding Minute gene encodes a translation initiation factor subunit. Significantly, MRP genes and more than 20 CRP genes do not correspond to Minute loci.This work answers a longstanding question about the molecular nature of Minute loci and suggests that Minute phenotypes arise from suboptimal protein synthesis resulting from reduced levels of cytoribosomes. Furthermore, by identifying the majority of haplolethal and haplosterile loci at the molecular level, our data will directly benefit efforts to attain complete deletion coverage of the D. melanogaster genome.

PubMed ID
PubMed Central ID
PMC2246290 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Correction to 'Minute status' of RpL26.
Cook and Marygold, 2015.10.1, Correction to 'Minute status' of RpL26. [FBrf0229855]

Df(2L)TW161 breakpoints.
Marygold and Cook, 2016.11.11, Df(2L)TW161 breakpoints. [FBrf0234092]

Correction to BSC119 deletion data in Marygold et al. 2007.
Cook and Marygold, 2017.5.24, Correction to BSC119 deletion data in Marygold et al. 2007. [FBrf0235614]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genome Biol.
    Title
    Genome Biology
    Publication Year
    2000-
    ISBN/ISSN
    1465-6906 1474-7596 1474-760X
    Data From Reference
    Aberrations (618)
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    Alleles (124)
    Gene Groups (4)
    Genes (280)
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    Insertions (9)
    Transgenic Constructs (1)