Reference Report

Reference
Citation	Beckervordersandforth, R.M., Rickert, C., Altenhein, B., Technau, G.M. (2008). Subtypes of glial cells in the Drosophila embryonic ventral nerve cord as related to lineage and gene expression. Mech. Dev. 125(5-6): 542--557. (Export to RIS)
FlyBase ID	FBrf0205499
Publication Type	Research paper
PubMed ID	18296030
PubMed Abstract	In the Drosophila embryonic CNS several subtypes of glial cells develop, which arrange themselves at characteristic positions and presumably fulfil specific functions. The mechanisms leading to the specification and differentiation of glial subtypes are largely unknown. By DiI labelling in glia-specific Gal4 lines we have clarified the lineages of the lateral glia in the embryonic ventral nerve cord and linked each glial cell to a specific stem cell. For the lineage of the longitudinal glioblast we show that it consists of 9 cells, which acquire at least four different identities. A large collection of molecular markers (many of them representing transcription factors and potential Gcm target genes) reveals that individual glial cells express specific combinations of markers. However, cluster analysis uncovers similar combinatorial codes for cells within, and significant differences between the categories of surface-associated, cortex-associated, and longitudinal glia. Glial cells derived from the same stem cell may be homogeneous (though not identical; stem cells NB1-1, NB5-6, NB6-4, LGB) or heterogeneous (NB7-4, NB1-3) with regard to gene expression. In addition to providing a powerful tool to analyse the fate of individual glial cells in different genetic backgrounds, each of these marker genes represents a candidate factor involved in glial specification or differentiation. We demonstrate this by the analysis of a castor loss of function mutation, which affects the number and migration of specific glial cells.
DOI	10.1016/j.mod.2007.12.004
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Alleles (25)
	Avic\GFP^{Stinger.Scer\UAS.T:nls-tra} cas^j1C2 Ecol\lacZ³¹⁰¹ Ecol\lacZ^AO75 Ecol\lacZ^{Ef1α48D-01275} Ecol\lacZ^en.Xba Ecol\lacZ^Fie-Y15 Ecol\lacZ^ftz.P Ecol\lacZ^fz3-J29 Ecol\lacZ^H15-H-15 Ecol\lacZ^hkb-5953 Ecol\lacZ^M84 Ecol\lacZ^mirr-P1 Ecol\lacZ^Mz813 Ecol\lacZ^P101 Ecol\lacZ^rL50 Ecol\lacZ^salm-03602 Ecol\lacZ^svp-3 Ecol\lacZ^wg-en11 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^cas-3921 Scer\GAL4^Eaat1.PR Scer\GAL4^Mz97 Scer\GAL4^nrv2.PS Scer\GAL4^repo
Constructs (9)
	P{Eaat1-GAL4.R} P{en-lacZ(ryXba)} P{ftz-lacZ.w⁺} P{GawB} P{lwB} P{nrv2-GAL4.S} P{PZ} P{UAS-mCD8::GFP.L} P{UAS-Stinger}
Genes (32)
	alrm Avic\GFP cas CG6218 CG7433 CG8965 CG9336 dpn Dr Ecol\lacZ en fabp ftz gcm gsb hkb lbe mirr Mmus\Cd8a moody Nep4 NimC4 pain pros repo salm Scer\GAL4 svp wg zfh1 zfh2 zyd
Insertions (18)
	P{A92}P101 P{en1}wg^en11 P{GAL4}cas³⁹²¹ P{GAL4}repo P{GawB}Mz97 P{GawB}Mz813 P{HZ}svp³ P{lacW}M84 P{lacW}mirr^P1 P{lacZ}3101 P{lacZ}Fie^Y15 P{lacZ}fz3^J29 P{lacZ-un4}hkb⁵⁹⁵³ P{lwB}AO75 P{PZ}Ef1α48D⁰¹²⁷⁵ P{PZ}H-15 P{PZ}rL50 P{PZ}salm⁰³⁶⁰²