Reference Report

Reference
Citation	Ratnaparkhi, G.S., Duong, H.A., Courey, A.J. (2008). Dorsal interacting protein 3 potentiates activation by Drosophila Rel homology domain proteins. Dev. Comp. Immunol. 32(11): 1290--1300. (Export to RIS)
FlyBase ID	FBrf0205502
Publication Type	Research paper
PubMed ID	18538389
PubMed Abstract	Dorsal interacting protein 3 (Dip3) contains a MADF DNA-binding domain and a BESS protein interaction domain. The Dip3 BESS domain was previously shown to bind to the Dorsal Rel homology domain. We show here that Dip3 also binds to the Relish Rel homology domain and enhances Rel family transcription factor function in both dorsoventral patterning and the immune response. While Dip3 is not essential, Dip3 mutations enhance the embryonic patterning defects that result from dorsal haplo-insufficiency, indicating that Dip3 may render dorsoventral patterning more robust. Dip3 is also required for optimal resistance to immune challenge since Dip3 mutant adults and larvae infected with bacteria have shortened lifetimes relative to infected wild-type flies. Furthermore, the mutant larvae exhibit significantly reduced expression of antimicrobial defense genes. Chromatin immunoprecipitation experiments in S2 cells indicate the presence of Dip3 at the promoters of these genes, and this binding requires the presence of Rel proteins at these promoters.
DOI	10.1016/j.dci.2008.04.006
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Comp. Immunol.
Title	Developmental and Comparative Immunology
Publication Year	1977-
ISBN/ISSN	0145-305X
Data from Reference
Aberrations (1)
	Df(2R)PC4
Alleles (9)
	Dip3¹ Dip3^{Scer\UAS.P\T.cRa} dl¹ dl^{Scer\UAS.P\T.cRa} Rel^E20 Scer\GAL4^Cg.PA Scer\GAL4^Lsp2.PH Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} spz⁴
Constructs (5)
	P{Cg-GAL4.A} P{Lsp2-GAL4.H} P{matα4-GAL-VP16} P{UASp-Dip3.R} P{UASp-dl.R}
Genes (12)
	CecA1 Dif Dip3 dl Dpt Drs Rel rho Scer\GAL4 sna spz twi
Natural transposons (1)
	P-element