Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Findlay, G.D., Yi, X., MacCoss, M.J., Swanson, W.J. (2008). Proteomics reveals novel Drosophila seminal fluid proteins transferred at mating. PLoS Biol. 6(7): e178. (Export to RIS) | ||
| FlyBase ID | FBrf0205571 | ||
| Publication Type | Research paper | ||
| PubMed ID | 18666829 | ||
| PubMed Abstract | Across diverse taxa, seminal fluid proteins (Sfps) transferred at mating affect the reproductive success of both sexes. Such reproductive proteins often evolve under positive selection between species; because of this rapid divergence, Sfps are hypothesized to play a role in speciation by contributing to reproductive isolation between populations. In Drosophila, individual Sfps have been characterized and are known to alter male sperm competitive ability and female post-mating behavior, but a proteomic-scale view of the transferred Sfps has been missing. Here we describe a novel proteomic method that uses whole-organism isotopic labeling to detect transferred Sfps in mated female D. melanogaster. We identified 63 proteins, which were previously unknown to function in reproduction, and confirmed the transfer of dozens of predicted Sfps. Relative quantification of protein abundance revealed that several of these novel Sfps are abundant in seminal fluid. Positive selection and tandem gene duplication are the prevailing forces of Sfp evolution, and comparative proteomics with additional species revealed lineage-specific changes in seminal fluid content. We also report a proteomic-based gene discovery method that uncovered 19 previously unannotated genes in D. melanogaster. Our results demonstrate an experimental method to identify transferred proteins in any system that is amenable to isotopic labeling, and they underscore the power of combining proteomic and evolutionary analyses to shed light on the complex process of Drosophila reproduction. | ||
| DOI | 10.1371/journal.pbio.0060178 | ||
| Related Publication(s) | |||
| Supplementary material | Table S6. Novel D. melanogaster Genes Identified by a Six-Frame Translation Database Search and Confirmed by RACE and RT-PCR. [FBrf0205906] Table S2. High-Confidence Transferred Sfps Detected in Canton S and tudor Experiments. [FBrf0208306] |
||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | Journal | ||
| Abbreviation | PLoS Biol. | ||
| Title | PLoS Biology | ||
| Publication Year | 2003- | ||
| ISBN/ISSN | 1545-7885 1544-9173 | ||
|
Data from Reference
|
|||
| Genes (18)
|
|||