Reference Report

Reference
Citation	Liu, Y., Lehmann, M. (2008). A genomic response to the yeast transcription factor GAL4 in Drosophila. Fly 2(2): 92--98. (Export to RIS)
FlyBase ID	FBrf0205590
Publication Type	Research paper
PubMed ID	18820459
PubMed Abstract	The yeast transcription factor GAL4 is widely used in Drosophila genetics to misexpress genes that are under control of the yeast upstream activator sequence (UAS). Here we show that high levels of GAL4 change the expression of many Drosophila genes in a UAS-independent manner, including genes that encode components of important signaling pathways. We find that at least part of the genomic response to GAL4 appears to be caused by effects of GAL4 on stress and immune response pathways. Finally, using the transcription factor Senseless as an example, we demonstrate how an interaction between GAL4 and a GAL4-driven protein can impede the use of the GAL4/UAS system in experiments aimed at determining the transcriptional response to a misexpressed gene.
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Secondary IDs	FBrf0209687
Language of Publication	English
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Publication Type	Journal
Abbreviation	Fly
Title	Fly
Publication Year	2007-
ISBN/ISSN	1933-6934 1933-6942
Data from Reference
Alleles (4)
	Scer\GAL4^hs.PB Scer\GAL4^Scer\UAS.cHa Scer\GAL4^αTub84B.PL sens^Scer\UAS.cNa
Constructs (4)
	P{GAL4-Hsp70.PB} P{tubP-GAL4} P{UAS-Gal4.H} P{UAS-sens.N}
Genes (88)
	ana Ark Atx-1 bin3 blow bowl Buffy by CG3277 CG3770 CG3909 CG4629 CG6023 CG7497 CG8108 CG9021 CG9449 CG9451 CG9452 CG9733 CG9776 CG11597 CG12825 CG13050 CG14292 CG14301 CG14949 CG15861 CG16890 CG17598 CG18128 CG18744 CG30062 CG31324 CG33127 CG34120 Chi cnn CR31781 crq Cyp4e2 debcl dl dos E(spl)mβ-HLH egr Eip78C Ets21C Fadd Fbp2 Fps85D HDAC6 hoe2 HP1b Ice Idgf3 Irk1 laza Mekk1 Mes-4 mof MTF-1 myo nAcRβ-21C os otu pcs per pie Pka-R1 Rpd3 Sce Scer\GAL4 sens shtd spz Tab2 Tdc1 th tim Tina-1 TpnC73F tra2 Traf4 upd2 upd3 Wnt6 ytr
Insertions (5)
	P{GAL4-Hsp70.PB}89-2-1 P{GAL4-Hsp70.PB}X1 P{tubP-GAL4}LL7 P{UAS-Gal4.H}12B P{UAS-sens.N}C5