Isolation and characterization of Df(1)BSC580
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC580 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f05811 and P{XP}d01801. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f05811/P{XP}d01801; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC580 from the segment of PBac{WH}f05811 to the left of its FRT site and the segment of P{XP}d01801 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d01801 to be Release 3 genomic coordinate 4428687 on the X chromosome. The Gene Disruption project determined the insertion site of P{XP}d01801 to be Release 3 genomic coordinate 4428693 on the X chromosome. This corresponds to 4C13 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f05811 on the Release 5 map is 4A5. Consequently, the cytological breakpoints of Df(1)BSC580 are predicted to be 4A5;4C13. It failed to complement bi1, rb1 and peb1.