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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2008.9.3). Isolation and characterization of Df(2R)BSC601. (Export to RIS) | ||
| FlyBase ID | FBrf0205617 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
From: Kevin Cook <kcook@XXXXXXXXXXXXXXX>
To: FlyBase-Cambridge <flybase-cambridgeXXXXXXXXXXXXXXX>, Kim Cook <ruacookXXXXXXXXXXXXXXX>, Stacey Christensen <sjchristXXXXXXXXXXXXXXX>, kaufmanXXXXXXXXXXXXXXX Subject: Isolation and characterization of Df(2R)BSC601 Date: Wed, 03 Sep 2008 11:58:42 -0400 (16:58 BST) Isolation and characterization of Df(2R)BSC601 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC601 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Nap1[f06242] and P{XP}d11424. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBac{WH}Nap1[f06242]/P{XP}d11424 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC601 from the segment of PBac{WH}Nap1[f06242] to the left of its FRT site and the segment of P{XP}d11424 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d11424 to be Release 3 genomic coordinate 19035686 on chromosome arm 2R. The Gene Disruption project determined the insertion site of P{XP}d11424 to be Release 3 genomic coordinate 19035692 on arm 2R. This corresponds to 60B1 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}Nap1[f06242] on the Release 5 map is 60A9. Consequently, the cytological breakpoints of Df(2R)BSC601 are predicted to be 60A9;60B1. Df(2R)BSC601 failed to complement Phm[k07623] and or[49h]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (2)
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| Constructs (1)
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| Genes (2)
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| Insertions (3)
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