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Christensen, S., Cook, K., Cook, K. (2008.9.3). Isolation and characterization of Df(2R)BSC610. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(2R)BSC610
Date: 	Wed, 03 Sep 2008  12:00:44  -0400  ( 17:00  BST)
Isolation and characterization of Df(2R)BSC610
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC610 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d05179 and PBac{WH}or[f06278]. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d05179/PBac{WH}or[f06278] males crossed 
to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC610 from the segment of P{XP}d05179 
to the left of its FRT site and the segment of PBac{WH}or[f06278] to 
the right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(2R)BSC610 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
59F6;60A14. Df(2R)BSC610 failed to complement ken[1], eIF6[k13214] and bgcn[1].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (1)
    Alleles (3)
    Genes (3)
    Insertions (3)
    Transgenic Constructs (1)