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Citation
Jensen, L.T., Nielsen, M.M., Loeschcke, V. (2008). New candidate genes for heat resistance in Drosophila melanogaster are regulated by HSF.  Cell Stress Chaperones 13(2): 177--182.
FlyBase ID
FBrf0205675
Publication Type
Research paper
Abstract

The cellular heat stress response is well studied in Drosophila in respect to the role of heat shock proteins (Hsp). Hsps are molecular chaperones, highly expressed during and after exposure to numerous stress types. Hsps are all regulated by a common transcription factor, the heat shock factor (HSF), and it is known that HSF is controlling other, so far uncharacterised, heat-responsive genes. In this study, we investigate whether novel candidate genes for heat resistance, identified by microarray experiments, are regulated by HSF. The microarray experiments recently identified several strongly upregulated genes in response to a short, non-lethal heat treatment in Drosophila melanogaster. To test whether or not a subset of these genes are HSF-induced, we studied 11 currently unannotated genes using quantitative polymerase chain reaction on HSF mutant flies with a non-functional HSF at elevated temperatures. We found indication of HSF regulation in most of the studied genes, suggesting a role of these unknown genes in heat tolerance. Surprisingly, some of the genes seemed to be upregulated independent of HSF function. The high induction in response to heat, which mimics the expression profile of Hsps, implies a role in the cellular heat response of these genes as well.

PubMed ID
PubMed Central ID
PMC2673890 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Stress Chaperones
    Title
    Cell Stress & Chaperones
    Publication Year
    1996-
    ISBN/ISSN
    1355-8145
    Data From Reference
    Genes (12)