Reference Report

Reference
Citation	Pfeiffer, B.D., Jenett, A., Hammonds, A.S., Ngo, T.T.B., Misra, S., Murphy, C., Scully, A., Carlson, J.W., Wan, K.H., Laverty, T.R., Mungall, C., Svirskas, R., Kadonaga, J.T., Doe, C.Q., Eisen, M.B., Celniker, S.E., Rubin, G.M. (2008). Tools for neuroanatomy and neurogenetics in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 105(28): 9715--9720. (Export to RIS)
FlyBase ID	FBrf0205679
Publication Type	Research paper
PubMed ID	18621688
PubMed Abstract	We demonstrate the feasibility of generating thousands of transgenic Drosophila melanogaster lines in which the expression of an exogenous gene is reproducibly directed to distinct small subsets of cells in the adult brain. We expect the expression patterns produced by the collection of 5,000 lines that we are currently generating to encompass all neurons in the brain in a variety of intersecting patterns. Overlapping 3-kb DNA fragments from the flanking noncoding and intronic regions of genes thought to have patterned expression in the adult brain were inserted into a defined genomic location by site-specific recombination. These fragments were then assayed for their ability to function as transcriptional enhancers in conjunction with a synthetic core promoter designed to work with a wide variety of enhancer types. An analysis of 44 fragments from four genes found that >80% drive expression patterns in the brain; the observed patterns were, on average, comprised of <100 cells. Our results suggest that the D. melanogaster genome contains >50,000 enhancers and that multiple enhancers drive distinct subsets of expression of a gene in each tissue and developmental stage. We expect that these lines will be valuable tools for neuroanatomy as well as for the elucidation of neuronal circuits and information flow in the fly brain.
DOI	10.1073/pnas.0803697105
Related Publication(s)
Supplementary material	Supporting Information: Construction of the Vectors pBDP, pBPGw, and pBPGUw. [FBrf0210340]
Personal communication to FlyBase	GAL4 Driver Collection of Rubin Laboratory at Janelia Farm. Pfeiffer et al., 2011.2.9, GAL4 Driver Collection of Rubin Laboratory at Janelia Farm. [FBrf0213040] P{GMR72E03-GAL4} construct and insertion. Rubin and Parks, 2011.7.27, P{GMR72E03-GAL4} construct and insertion. [FBrf0214515] GAL4 Driver Collection of Rubin Laboratory at Janelia Farm - Additional Lines. Pfeiffer et al., 2011.11.21, GAL4 Driver Collection of Rubin Laboratory at Janelia Farm - Additional Lines. [FBrf0216821] Janelia GAL4 Drivers - adult brain annotation table. Jenett and Svirskas, 2012.9.24, Janelia GAL4 Drivers - adult brain annotation table. [FBrf0219498]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (51)
	Avic\GFP^Scer\UAS.cUa Scer\GAL4^GMR1A01 Scer\GAL4^GMR1A02 Scer\GAL4^GMR1A03 Scer\GAL4^GMR9B04 Scer\GAL4^GMR9B05.EP Scer\GAL4^GMR9B05 Scer\GAL4^GMR9B06 Scer\GAL4^GMR9B07 Scer\GAL4^GMR9B08 Scer\GAL4^GMR9B09 Scer\GAL4^GMR9B10 Scer\GAL4^GMR9C02 Scer\GAL4^GMR9C06 Scer\GAL4^GMR9C07 Scer\GAL4^GMR9C08 Scer\GAL4^GMR9C09 Scer\GAL4^GMR9C10 Scer\GAL4^GMR9C11.EP Scer\GAL4^GMR9C11 Scer\GAL4^GMR9C12 Scer\GAL4^GMR9D02 Scer\GAL4^GMR9D03 Scer\GAL4^GMR9D04 Scer\GAL4^GMR9D05 Scer\GAL4^GMR9D06.EP Scer\GAL4^GMR9D08 Scer\GAL4^GMR9D09 Scer\GAL4^GMR9D10 Scer\GAL4^GMR9D11 Scer\GAL4^GMR9G07 Scer\GAL4^GMR9G08 Scer\GAL4^GMR9G09 Scer\GAL4^GMR9G10 Scer\GAL4^GMR9H01 Scer\GAL4^GMR9H02 Scer\GAL4^GMR9H03 Scer\GAL4^GMR9H04 Scer\GAL4^GMR9H06 Scer\GAL4^GMR9H07 Scer\GAL4^GMR9H08 Scer\GAL4^GMR9H09 Scer\GAL4^GMR9H10 Scer\GAL4^GMR9H11 Scer\GAL4^GMR9H12 Scer\GAL4^GMR19H07 Scer\GAL4^GMR20C11 Scer\GAL4^GMR20E11 Scer\GAL4^GMR21E03 Scer\GAL4^GMR62A03.EP w^+mC
Constructs (52)
	P{GMR1A01-GAL4} P{GMR1A02-GAL4} P{GMR1A03-GAL4} P{GMR9B04-GAL4} P{GMR9B05-GAL4.EP} P{GMR9B05-GAL4} P{GMR9B06-GAL4} P{GMR9B07-GAL4} P{GMR9B08-GAL4} P{GMR9B09-GAL4} P{GMR9B10-GAL4} P{GMR9C02-GAL4} P{GMR9C06-GAL4} P{GMR9C07-GAL4} P{GMR9C08-GAL4} P{GMR9C09-GAL4} P{GMR9C10-GAL4} P{GMR9C11-GAL4.EP} P{GMR9C11-GAL4} P{GMR9C12-GAL4} P{GMR9D02-GAL4} P{GMR9D03-GAL4} P{GMR9D04-GAL4} P{GMR9D05-GAL4} P{GMR9D06-GAL4.EP} P{GMR9D08-GAL4} P{GMR9D09-GAL4} P{GMR9D10-GAL4} P{GMR9D11-GAL4} P{GMR9G07-GAL4} P{GMR9G08-GAL4} P{GMR9G09-GAL4} P{GMR9G10-GAL4} P{GMR9H01-GAL4} P{GMR9H02-GAL4} P{GMR9H03-GAL4} P{GMR9H04-GAL4} P{GMR9H06-GAL4} P{GMR9H07-GAL4} P{GMR9H08-GAL4} P{GMR9H09-GAL4} P{GMR9H10-GAL4} P{GMR9H11-GAL4} P{GMR9H12-GAL4} P{GMR19H07-GAL4} P{GMR20C11-GAL4} P{GMR20E11-GAL4} P{GMR21E03-GAL4} P{GMR62A03-GAL4.EP} P{UAS-GFP.U} pBPGUw pBPGw
Genes (12)
	Avic\GFP CG4580 CG33673 dac erm Idgf3 oa2 plexA Scer\GAL4 sphinx toy tRNA:CR31942
Insertions (10)
	P{CaryP}attP2 P{GMR9B05-GAL4.EP}attP2 P{GMR9C11-GAL4.EP}attP2 P{GMR9D05-GAL4}attP P{GMR9D06-GAL4.EP}attP2 P{GMR19H07-GAL4}attP2 P{GMR20C11-GAL4}attP2 P{GMR20E11-GAL4}attP2 P{GMR21E03-GAL4}attP2 P{GMR62A03-GAL4.EP}attP2
Libraries/Collections (1)
	GMR_Brain_exp_1
Natural transposons (1)
	P-element
Sequence features (16)
	CG31760_R9D03 CG31760_R9D06 CG31760_R9D10 CG31760_R9D11 dac_R9B05 dac_R9B07 dac_R9C11 oa2_R19H07 oa2_R20C11 oa2_R20E11 toy_R9A02 toy_R9G08 toy_R9G09 toy_R9G10 toy_R9H01 toy_R9H03