Reference Report

Reference
Citation	Moon, N.S., Di Stefano, L., Morris, E.J., Patel, R., White, K., Dyson, N.J. (2008). E2F and p53 induce apoptosis independently during Drosophila development but intersect in the context of DNA damage. PLoS Genet. 4(8): e1000153. (Export to RIS)
FlyBase ID	FBrf0205772
Publication Type	Research paper
PubMed ID	18688282
PubMed Abstract	In mammalian cells, RB/E2F and p53 are intimately connected, and crosstalk between these pathways is critical for the induction of cell cycle arrest or cell death in response to cellular stresses. Here we have investigated the genetic interactions between RBF/E2F and p53 pathways during Drosophila development. Unexpectedly, we find that the pro-apoptotic activities of E2F and p53 are independent of one another when examined in the context of Drosophila development: apoptosis induced by the deregulation of dE2F1, or by the overexpression of dE2F1, is unaffected by the elimination of dp53; conversely, dp53-induced phenotypes are unaffected by the elimination of dE2F activity. However, dE2F and dp53 converge in the context of a DNA damage response. Both dE2F1/dDP and dp53 are required for DNA damage-induced cell death, and the analysis of rbf1 mutant eye discs indicates that dE2F1/dDP and dp53 cooperatively promote cell death in irradiated discs. In this context, the further deregulation in the expression of pro-apoptotic genes generates an additional sensitivity to apoptosis that requires both dE2F/dDP and dp53 activity. This sensitivity differs from DNA damage-induced apoptosis in wild-type discs (and from dE2F/dDP-induced apoptosis in un-irradiated rbf1 mutant eye discs) by being dependent on both hid and reaper. These results show that pro-apoptotic activities of dE2F1 and dp53 are surprisingly separable: dp53 is required for dE2F-dependent apoptosis in the response to DNA damage, but it is not required for dE2F-dependent apoptosis caused simply by the inactivation of rbf1.
DOI	10.1371/journal.pgen.1000153
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	PLoS Genet.
Title	PLoS Genetics
Publication Year	2005-
ISBN/ISSN	1553-7404 1553-7390
Data from Reference
Aberrations (1)
	Df(3L)X14
Alleles (17)
	Dp^a2 Dp^a4 Dp^Scer\UAS.cDa Dp^unspecified E2f^Scer\UAS.cNa p53^5A-1-4 p53^259H.GUS p53^GUS.PB Rbf^120a Rbf^Scer\UAS.cDa Rbf^Δ14 rpr⁸⁷ Scer\GAL4^Act88F.PD Scer\GAL4^GMR.PF Scer\GAL4^nos.PG Scer\GAL4^sca-T3 W⁰⁵⁰¹⁴
Constructs (8)
	P{Act88F-GAL4.D} P{GAL4-ninaE.GMR} P{GAL4-nos.NGT} P{GUS-p53.259H} P{GUS-p53} P{UAS-Dp.D} P{UAS-E2f.N} P{UAS-Rbf.D}
Genes (8)
	Dp E2f p53 Rbf RnrS rpr Scer\GAL4 W
Insertions (2)
	P{GawB}sca^T3 P{SUPor-P}KG07184