Reference Report

Reference
Citation	Takashima, S., Mkrtchyan, M., Younossi-Hartenstein, A., Merriam, J.R., Hartenstein, V. (2008). The behaviour of Drosophila adult hindgut stem cells is controlled by Wnt and Hh signalling. Nature 454(7204): 651--655. (Export to RIS)
FlyBase ID	FBrf0205829
Publication Type	Research paper
PubMed ID	18633350
PubMed Abstract	The intestinal tract maintains proper function by replacing aged cells with freshly produced cells that arise from a population of self-renewing intestinal stem cells (ISCs). In the mammalian intestine, ISC self renewal, amplification and differentiation take place along the crypt-villus axis, and are controlled by the Wnt and hedgehog (Hh) signalling pathways. However, little is known about the mechanisms that specify ISCs within the developing intestinal epithelium, or about the signalling centres that help maintain them in their self-renewing stem cell state. Here we show that in adult Drosophila melanogaster, ISCs of the posterior intestine (hindgut) are confined to an anterior narrow segment, which we name the hindgut proliferation zone (HPZ). Within the HPZ, self renewal of ISCs, as well as subsequent proliferation and differentiation of ISC descendants, are controlled by locally emanating Wingless (Wg, a Drosophila Wnt homologue) and Hh signals. The anteriorly restricted expression of Wg in the HPZ acts as a niche signal that maintains cells in a slow-cycling, self-renewing mode. As cells divide and move posteriorly away from the Wg source, they enter a phase of rapid proliferation. During this phase, Hh signal is required for exiting the cell cycle and the onset of differentiation. The HPZ, with its characteristic proliferation dynamics and signalling properties, is set up during the embryonic phase and becomes active in the larva, where it generates all adult hindgut cells including ISCs. The mechanism and genetic control of cell renewal in the Drosophila HPZ exhibits a large degree of similarity with what is seen in the mammalian intestine. Our analysis of the Drosophila HPZ provides an insight into the specification and control of stem cells, highlighting the way in which the spatial pattern of signals that promote self renewal, growth and differentiation is set up within a genetically tractable model system.
DOI	10.1038/nature07156
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Alleles (11)
	Avic\GFP^{S65T.Scer\UAS} Avic\GFP^Stat92E.10X ci^{Ce-2.Scer\UAS.T:Ivir\HA1} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} pan^ΔN.Scer\UAS Scer\GAL4^byn-Gal4 Scer\GAL4^hh-Gal4 Scer\GAL4^wg.PM Scer\GAL80^ts.αTub84B Scer\GAL80^αTub84B.PL wg^{Scer\UAS.cHa.T:Ivir\HA1}
Constructs (9)
	P{10XStat92E-GFP} P{GAL4-wg.M} P{tubP-GAL80} P{tubP-GAL80^ts} P{UAS-ci.Ce-2.HA} P{UAS-GFP.S65T} P{UAS-mCD8::GFP.L} P{UAS-pan.dTCFΔN} P{UAS-wg.H.T:HA1}
Genes (11)
	arm Avic\GFP byn ci hh Mmus\Cd8a pan Scer\GAL4 Scer\GAL80 Stat92E wg
Insertions (2)
	P{GAL4}hh^Gal4 P{GawB}byn^Gal4