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Tian, C., Gao, B., Rodriguez, M.C., Lanz-Mendoza, H., Ma, B., Zhu, S. (2008). Gene expression, antiparasitic activity, and functional evolution of the drosomycin family.  Molec. Immunol. 45(15): 3909--3916.
FlyBase ID
FBrf0205847
Publication Type
Research paper
Abstract

Drosophila employs various antimicrobial peptides as effective weapons to defend against diverse pathogens. Drosomycin is an inducible antifungal peptide initially isolated from the Drosophila melanogaster haemolymph. Here we report the expression pattern of seven drosomycin genes in four different developmental stages (egg, larva, pupa and adult). Results show that drosomycin and drosomycin-2 are expressed in larva, pupa and adult, whereas drosomycin-1 and drosomycin-6 were not detected in all the stages. Moreover, all the seven drosomycin genes are shut off in egg. Functional comparison of recombinant drosomycin and drosomycin-2, both with identical expression pattern, produced from Escherichia coli, revealed their significant differences in potency against a specific fungal species. In addition, we found for the first time that drosomycin and drosomycin-2 both are antiparasitic peptides which show inhibitory effect on the ookinete development of the parasite Plasmodium berghei with differential potency. Functional differentiation between them was further evaluated by evolutionary trace analysis which identified two evolutionary epitopes (named alpha- and gamma-patch, respectively) and an important site in the m-loop. Substitutions in these regions are possibly associated with the antifungal and antiparasitic potency difference among members of the drosomycin family.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Molec. Immunol.
    Title
    Molecular Immunology
    Publication Year
    1979-
    ISBN/ISSN
    0161-5890
    Data From Reference
    Gene Groups (1)
    Genes (7)