Reference Report

Reference
Citation	Kaplan, D.D., Zimmermann, G., Suyama, K., Meyer, T., Scott, M.P. (2008). A nucleostemin family GTPase, NS3, acts in serotonergic neurons to regulate insulin signaling and control body size. Genes Dev. 22(14): 1877--1893. (Export to RIS)
FlyBase ID	FBrf0205884
Publication Type	Research paper
PubMed ID	18628395
PubMed Abstract	Growth and body size are regulated by the CNS, integrating the genetic developmental program with assessments of an animal's current energy state and environmental conditions. CNS decisions are transmitted to all cells of the animal by insulin/insulin-like signals. The molecular biology of the CNS growth control system has remained, for the most part, elusive. Here we identify NS3, a Drosophila nucleostemin family GTPase, as a powerful regulator of body size. ns3 mutants reach <60% of normal size and have fewer and smaller cells, but exhibit normal body proportions. NS3 does not act cell-autonomously, but instead acts at a distance to control growth. Rescue experiments were performed by expressing wild-type ns3 in many different cells of ns3 mutants. Restoring NS3 to only 106 serotonergic neurons rescued global growth defects. These neurons are closely apposed with those of insulin-producing neurons, suggesting possible communication between the two neuronal systems. In the brains of ns3 mutants, excess serotonin and insulin accumulate, while peripheral insulin pathway activation is low. Peripheral insulin pathway activation rescues the growth defects of ns3 mutants. The findings suggest that NS3 acts in serotonergic neurons to regulate insulin signaling and thus exert global growth control.
DOI	10.1101/gad.1670508
Related Publication(s)
Supplementary material	Supplemental Research Data. [FBrf0207883]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (24)
	Akt1^{T342D.S505D.GMR} Avic\GFP^Scer\UAS.cUa Ns3^G0431 Ns3^{Scer\UAS.T:Avic\GFP-YFP.Venus} Scer\GAL4^2-286 Scer\GAL4^71B Scer\GAL4^386Y Scer\GAL4^Cha.7.4 Scer\GAL4^Ddc.PL Scer\GAL4^dimm-929 Scer\GAL4^elav.PLu Scer\GAL4^en-e16E Scer\GAL4^Ilp2.PR Scer\GAL4^Lsp2.PH Scer\GAL4^npf.1 Scer\GAL4^NPFR1.6.6 Scer\GAL4^Orco.7.087 Scer\GAL4^ple.PF Scer\GAL4^repo Scer\GAL4^S3.hug Scer\GAL4^Tdc2.PC Scer\GAL4^tub.PU T:Avic\GFP^YFP.Venus Tor^ΔP
Constructs (15)
	P{Cha-GAL4.7.4} P{Ddc-GAL4.L} P{GAL4-elav.L} P{GMR-Akt1.T342D.S505D} P{hug-GAL4.S3} P{Ilp2-GAL4.R} P{Lsp2-GAL4.H} P{npf-GAL4.1} P{NPFR1-GAL4.6.6} P{Orco-GAL4.7.087} P{ple-GAL4.F} P{Tdc2-GAL4.C} P{tub-GAL4.U} P{UAS-GFP.U} P{UAS-ns3.YFP}
Genes (14)
	Akt1 Avic\GFP CG14777 CG14786 CG14787 Ilp2 Ns1 Ns2 Ns3 Ns4 O-fut2 RpS6 Scer\GAL4 Tor
Insertions (6)
	P{GAL4}repo P{GawB}2-286 P{GawB}71B P{GawB}386Y P{GawB}dimm⁹²⁹ P{lacW}Ns3^G0431