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Christensen, S., Cook, K., Cook, K. (2008.9.29). Isolation and characterization of Df(1)BSC626. 
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FBrf0205925
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From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(1)BSC626
Date: 	Mon, 29 Sep 2008  15:38:01  -0400  ( 20:38  BST)
Isolation and characterization of Df(1)BSC626
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC626 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d08010 and PBac{WH}f07352. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{XP}d08010/PBac{WH}f07352; MKRS, P{hsFLP}86E/+ females crossed to 
Binsinscy/Y males. These females were heat shocked as larvae as 
described in Parks et al., Nature Genetics 36: 288-292, 2004 
(FBrf0175003). This cross and crosses in preceding and succeeding 
generations maintained the original genetic background of the 
Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC626 from the segment of P{XP}d08010 
to the left of its FRT site and the segment of PBac{WH}f07352 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(1)BSC626 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
19E1;19F4. It failed to complement run[2].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    English
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)