Isolation and characterization of Df(3R)BSC619
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC619 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG6697f06785 and P{XP}d05036. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG6697f06785/P{XP}d05036 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC619 from the segment of PBac{WH}CG6697f06785 to the left of its FRT site and the segment of P{XP}d05036 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC619 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 94D10;94E13. Df(3R)BSC619 failed to complement hh21 and cnc03921.