Reference Report

Reference
Citation	Tien, A.C., Rajan, A., Schulze, K.L., Ryoo, H.D., Acar, M., Steller, H., Bellen, H.J. (2008). Ero1L, a thiol oxidase, is required for Notch signaling through cysteine bridge formation of the Lin12-Notch repeats in Drosophila melanogaster. J. Cell Biol. 182(6): 1113--1125. (Export to RIS)
FlyBase ID	FBrf0206028
Publication Type	Research paper
PubMed ID	18809725
PubMed Abstract	Notch-mediated cell-cell communication regulates numerous developmental processes and cell fate decisions. Through a mosaic genetic screen in Drosophila melanogaster, we identified a role in Notch signaling for a conserved thiol oxidase, endoplasmic reticulum (ER) oxidoreductin 1-like (Ero1L). Although Ero1L is reported to play a widespread role in protein folding in yeast, in flies Ero1L mutant clones show specific defects in lateral inhibition and inductive signaling, two characteristic processes regulated by Notch signaling. Ero1L mutant cells accumulate high levels of Notch protein in the ER and induce the unfolded protein response, suggesting that Notch is misfolded and fails to be exported from the ER. Biochemical assays demonstrate that Ero1L is required for formation of disulfide bonds of three Lin12-Notch repeats (LNRs) present in the extracellular domain of Notch. These LNRs are unique to the Notch family of proteins. Therefore, we have uncovered an unexpected requirement for Ero1L in the maturation of the Notch receptor.
DOI	10.1083/jcb.200805001
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(1)N-54l9
Alleles (20)
	CG4670^GD1978 Dl^{Scer\UAS.T:Hsap\MYC} Egfr^E1 Ero1L^23T Ero1L^335QRS Ero1L^B3 Ero1L^C8 Ero1L^GD3577 Ero1L^Scer\UAS.cTa Hsap\ERO1LB^{C394A.Lα.Scer\UAS} Hsap\ERO1LB^Lα.Scer\UAS Hsc70-3^{D231S.Scer\UAS} N^Ax-E2 N^ECN.Scer\UAS Scer\GAL4^Act.PU Scer\GAL4^da.G32 Scer\GAL4^nub.PK Scer\GAL4^ptc-559.1 Scer\GAL4^αTub84B.PL Scer\GAL80^αTub84B.PL
Constructs (13)
	P{Act-GAL4.U} P{GAL4-da.G32} P{GD1978} P{GD3577} P{nub-GAL4.K} P{tubP-GAL4} P{tubP-GAL80} P{UAS(-FRT)Dl.T:Hsap\MYC} P{UAS-Ero1L.T} P{UAS-hERO1-Lα.C394A} P{UAS-hERO1-Lα} P{UAS-Hsc70-3.D231S} P{UAS-N.ECN}
Genes (19)
	boca CG4670 ci crb ct Dl Egfr elav Ero1L Fas2 Hsap\ERO1LB Hsc70-3 Mad N Scer\GAL4 Scer\GAL80 sens vg wg
Insertions (1)
	P{GawB}ptc^559.1
Natural transposons (1)
	P-element