Isolation and characterization of Df(3R)BSC634
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC634 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f02639 and P{XP}d05521. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f02639/P{XP}d05521 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC634 from the segment of PBac{WH}f02639 to the left of its FRT site and the segment of P{XP}d05521 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC634 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 90B3;90B7. Df(3R)BSC634 failed to complement l(3)0788207882 for female sterility and Df(3R)ED5780 for lethality.