Isolation and characterization of Df(1)BSC646
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC646 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06197 and PBac{WH}f07805. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}d06197/PBac{WH}f07805; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC646 from the segment of P{XP}d06197 to the left of its FRT site and the segment of PBac{WH}f07805 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d06197 to be Release 3 genomic coordinate 20003203 on the X chromosome. This corresponds to 19C3 on the Release 3 genome map and 19C4 on the Release 5 genome map. The predicted position of PBac{WH}f07805 on the Release 5 map is 19E4. Consequently, the cytological breakpoints of Df(1)BSC646 are predicted to be 19C4;19E4. It failed to complement Df(1)Exel6254 and Df(1)HM44.