Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Christensen, S., Cook, K., Cook, K. (2008.10.20). Isolation and characterization of Df(1)BSC647. (Export to RIS) | ||
| FlyBase ID | FBrf0206124 | ||
| Publication Type | Personal communication to FlyBase | ||
| PubMed ID | |||
| PubMed Abstract | |||
| Text of Personal Communication |
From: Kevin Cook <kcook@XXXXXXXXXXXXXXX>
Subject: Isolation and characterization of Df(1)BSC647 Date: Mon, 20 Oct 2008 09:19:51 -0400 (14:19 BST) Isolation and characterization of Df(1)BSC647 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC647 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03971 and P{XP}CG32552d09154. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f03971/P{XP}CG32552d09154; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC647 from the segment of PBac{WH}f03971 to the left of its FRT site and the segment of P{XP}CG32552d09154 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f03971 to be Release 3 genomic coordinate 17442428 on the X chromosome, which corresponds to 16C3 on the Release 3 genome map and 16C1 on the Release 5 genome map. Exelixis, Inc. determined the insertion site of P{XP}CG32552d09154 to be Release 3 genomic coordinate 17794134 on the X chromosome, which corresponds to 16F6 on both the Release 3 and Release 5 genome maps. Consequently, the cytological breakpoints of Df(1)BSC647 are predicted to be 16C1;16F6. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX |
||
| DOI | |||
| Related Publication(s) | |||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | |||
| Abbreviation | |||
| Title | |||
| ISBN/ISSN | |||
|
Data from Reference
|
|||
| Aberrations (1)
|
|||
| Constructs (1)
|
|||
| Insertions (3)
|
|||