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Christensen, S., Cook, K., Cook, K. (2008.10.20). Isolation and characterization of Df(3R)BSC650. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, kaufmanXXXX, Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>
Subject: 	Isolation and characterization of Df(3R)BSC650
Date: 	Mon, 20 Oct 2008  09:20:30  -0400  ( 14:20  BST)
Isolation and characterization of Df(3R)BSC650
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC650 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}CG17801e02870 and P{XP}d01767. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to 
P{hsFLP}1, y1 w1118; PBac{RB}CG17801e02870/P{XP}d01767 males. 
The males were heat shocked as larvae as described in Parks et al., 
Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.RB3}BSC650 from the segment of PBac{RB}CG17801e02870 to 
the left of its FRT site and the segment of P{XP}d01767 to the right 
of its FRT site. Its presence was verified using the PCR methods and 
primers described in Parks et al. with the substitution of the primer 
5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus 
primer in the Hybrid PCR protocol in the Supplementary Methods. 
Exelixis, Inc. determined the insertion site of P{XP}d01767 to be 
Release 3 genomic coordinate 14124138 on chromosome arm 3R. This 
corresponds to 91A2 on the Release 3 and Release 5 genome maps. The 
predicted position of PBac{RB}CG17801e02870 on the Release 5 map is 
90C6. Consequently, the cytological breakpoints of Df(3R)BSC650 are 
predicted to be 90C6;91A2. Df(3R)BSC650 failed to complement 
cpo01432 and MED17s2956.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)