Open Close
Xu, R., Deng, K., Zhu, Y., Wu, Y., Ren, J., Wan, M., Zhao, S., Wu, X., Han, M., Zhuang, Y., Xu, T. (2008). A large-scale functional approach to uncover human genes and pathways in Drosophila.  Cell Res. 18(11): 1114--1127.
FlyBase ID
Publication Type
Research paper

We demonstrate the feasibility of performing a systematic screen for human gene functions in Drosophila by assaying for their ability to induce overexpression phenotypes. Over 1 500 transgenic fly lines corresponding to 236 human genes have been established. In all, 51 lines are capable of eliciting a phenotype suggesting that the human genes are functional. These heterologous genes are functionally relevant as we have found a similar mutant phenotype caused either by a dominant negative mutant form of the human ribosomal protein L8 gene or by RNAi downregulation of the Drosophila RPL8. Significantly, the Drosophila RPL8 mutant can be rescued by wild-type human RPL8. We also provide genetic evidence that Drosophila RPL8 is a new member of the insulin signaling pathway. In summary, the functions of many human genes appear to be highly conserved, and the ability to identify them in Drosophila represents a powerful genetic tool for large-scale analysis of human transcripts in vivo.

PubMed ID
PubMed Central ID
Related Publication(s)

Making brundlefly, one gene at a time.
Zhang et al., 2009, Cell Res. 19(1): 5--7 [FBrf0215375]

Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Cell Res.
    Cell Research
    Publication Year
    Data From Reference