Open Close
Reference
Citation
Xu, R., Deng, K., Zhu, Y., Wu, Y., Ren, J., Wan, M., Zhao, S., Wu, X., Han, M., Zhuang, Y., Xu, T. (2008). A large-scale functional approach to uncover human genes and pathways in Drosophila.  Cell Res. 18(11): 1114--1127.
FlyBase ID
FBrf0206163
Publication Type
Research paper
Abstract

We demonstrate the feasibility of performing a systematic screen for human gene functions in Drosophila by assaying for their ability to induce overexpression phenotypes. Over 1 500 transgenic fly lines corresponding to 236 human genes have been established. In all, 51 lines are capable of eliciting a phenotype suggesting that the human genes are functional. These heterologous genes are functionally relevant as we have found a similar mutant phenotype caused either by a dominant negative mutant form of the human ribosomal protein L8 gene or by RNAi downregulation of the Drosophila RPL8. Significantly, the Drosophila RPL8 mutant can be rescued by wild-type human RPL8. We also provide genetic evidence that Drosophila RPL8 is a new member of the insulin signaling pathway. In summary, the functions of many human genes appear to be highly conserved, and the ability to identify them in Drosophila represents a powerful genetic tool for large-scale analysis of human transcripts in vivo.

PubMed ID
PubMed Central ID
Related Publication(s)
Note

Making brundlefly, one gene at a time.
Zhang et al., 2009, Cell Res. 19(1): 5--7 [FBrf0215375]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Res.
    Title
    Cell Research
    Publication Year
    1990
    ISBN/ISSN
    1001-0602
    Data From Reference