Reference Report

Reference
Citation	McDonald, J.A., Khodyakova, A., Aranjuez, G., Dudley, C., Montell, D.J. (2008). PAR-1 Kinase Regulates Epithelial Detachment and Directional Protrusion of Migrating Border Cells. Curr. Biol. 18(21): 1659--1667. (Export to RIS)
FlyBase ID	FBrf0206227
Publication Type	Research paper
PubMed ID	18976916
PubMed Abstract	Many cells that migrate during normal embryonic development or in metastatic cancer first detach from an epithelium. However, this step is often difficult to observe directly in vivo, and the mechanisms controlling the ability of cells to leave the epithelium are poorly understood. In addition, once cells detach, they must assume a migratory phenotype, involving changes in cytoskeletal and signaling dynamics. Drosophila border cells provide a model system in which a combination of forward genetics and live-cell imaging can allow researchers to investigate the cellular and molecular mechanisms of epithelial cell detachment and migration in vivo.We identified the Drosophila homolog of the serine/threonine kinase PAR-1 (MARK/Kin1) in a screen for mutations that disrupt border cell migration. Previous studies identified two proteins, Apontic and Notch, that indirectly affect border cell detachment by regulating transcription of downstream targets. In contrast, PAR-1 directly modulates apical-basal polarity between border cells and epithelial cells to promote detachment. Furthermore, PAR-1, but not the apical polarity complex protein PAR-3, promotes the directionality of transient cell protrusions, which border cells require for sensing the chemoattractant gradient.We conclude that PAR-1-dependent apical-basal polarity is required for proper detachment of migratory border cells from neighboring epithelial cells. Moreover, polarity controlled by PAR-1 influences the ability of migratory cells to sense direction, a critical feature of migration. Thus, this work reveals new insights into two distinct, but essential, steps of epithelial cell migration.
DOI	10.1016/j.cub.2008.09.041
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (3)
	Df(2R)AA21 Df(2R)BSC22 Df(2R)BSC26
Alleles (21)
	14-3-3ζ^P1188 baz^GD1384 baz^{S151A.S1085A.Scer\UAS.P\T.T:Avic\GFP} baz^{Scer\UAS.P\T.T:Avic\GFP} Jupiter^{Scer\UAS.T:Disc\RFP-mCherry} lkb1^4A4-2 lkb1^4B1-11 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} par-1^27C1 par-1^36G4 par-1^42D9 par-1^70D3 par-1^{dsRNA.Scer\UAS.cMDa} par-1^k06323 par-1^{Scer\UAS.C*.T:Avic\GFP} par-1^W3 par-1^Δ-16 Scer\GAL4^Act5C.PI Scer\GAL4^c306 Scer\GAL4^slbo.2.6 T:Disc\RFP^mCherry
Constructs (8)
	P{GAL4-slbo.2.6} P{GD1384} P{UAS-mCD8::GFP.L} P{UAS-mCherry-Jupiter} P{UAS-par-1.C*.GFP} P{UAS-par-1.RNAi.MD} P{UASp-baz.GFP} P{UASp-baz.S151A.S1085A}
Genes (14)
	14-3-3ζ aPKC arm baz Fak Fas3 Jupiter lkb1 Mmus\Cd8a par-1 Scer\GAL4 shg sn Stat92E
Insertions (1)
	P{GawB}c306
Natural transposons (1)
	P-element