Reference Report

Reference
Citation	Von Stetina, J.R., Tranguch, S., Dey, S.K., Lee, L.A., Cha, B., Drummond-Barbosa, D. (2008). α-Endosulfine is a conserved protein required for oocyte meiotic maturation in Drosophila. Development 135(22): 3697--3706. (Export to RIS)
FlyBase ID	FBrf0206240
Publication Type	Research paper
PubMed ID	18927152
PubMed Abstract	Meiosis is coupled to gamete development and must be well regulated to prevent aneuploidy. During meiotic maturation, Drosophila oocytes progress from prophase I to metaphase I. The molecular factors controlling meiotic maturation timing, however, are poorly understood. We show that Drosophila alpha-endosulfine (endos) plays a key role in this process. endos mutant oocytes have a prolonged prophase I and fail to progress to metaphase I. This phenotype is similar to that of mutants of cdc2 (synonymous with cdk1) and of twine, the meiotic homolog of cdc25, which is required for Cdk1 activation. We found that Twine and Polo kinase levels are reduced in endos mutants, and identified Early girl (Elgi), a predicted E3 ubiquitin ligase, as a strong Endos-binding protein. In elgi mutant oocytes, the transition into metaphase I occurs prematurely, but Polo and Twine levels are unaffected. These results suggest that Endos controls meiotic maturation by regulating Twine and Polo levels, and, independently, by antagonizing Elgi. Finally, germline-specific expression of the human alpha-endosulfine ENSA rescues the endos mutant meiotic defects and infertility, and alpha-endosulfine is expressed in mouse oocytes, suggesting potential conservation of its meiotic function.
DOI	10.1242/dev.025114
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (3)
	Df(2L)RA5 Df(3L)brm11 Df(3L)ED4536
Alleles (16)
	cdc2^B47 cdc2^E1-24 dj^{T:Avic\GFP-S65T} elgi¹ elgi² endos⁰⁰⁰⁰³ endos^EY01105 endos^{Scer\UAS.P\T.cVSa} Hsap\ENSA^{Scer\UAS.P\T.cVSa} polo^{Scer\UAS.P\T.cXa} Scer\GAL4^{nos.UTR.T:Hsim\VP16} twe¹ twe^hs.PVS twe^{Scer\UAS.P\T.T:Hsap\MYC} twe^T:Ecol\lacZ w^+mC
Constructs (8)
	P{dj-GFP.S} P{GAL4::VP16-nos.UTR} P{hs-twe.VS} P{twe-lacZ} P{UASp-endos.VS} P{UASp-ENSA.VS} P{UASp-myc-twe} P{UASp-polo.X}
Genes (14)
	cdc2 CG9014 CG10916 CycB dj elgi endos Hsap\ENSA nopo polo Scer\GAL4 T:Hsap\MYC twe w
Insertions (3)
	P{EPgy2}EY10782 P{UASp-endos.VS}1 P{UASp-endos.VS}10
Natural transposons (1)
	P-element