Reference Report

Reference
Citation	Moyer, K.E., Jacobs, J.R. (2008). Varicose: a MAGUK required for the maturation and function of Drosophila septate junctions. BMC Dev. Biol. 8(): 99. (Export to RIS)
FlyBase ID	FBrf0206241
Publication Type	Research paper
PubMed ID	18847477
PubMed Abstract	Scaffolding proteins belonging to the membrane associated guanylate kinase (MAGUK) superfamily function as adapters linking cytoplasmic and cell surface proteins to the cytoskeleton to regulate cell-cell adhesion, cell-cell communication and signal transduction. We characterize here a Drosophila MAGUK member, Varicose (Vari), the homologue of vertebrate scaffolding protein PALS2.Varicose localizes to pleated septate junctions (pSJs) of all embryonic, ectodermally-derived epithelia and peripheral glia. In vari mutants, essential SJ proteins NeurexinIV and FasciclinIII are mislocalized basally and epithelia develop a leaky paracellular seal. In addition, vari mutants display irregular tracheal tube diameters and have reduced lumenal protein accumulation, suggesting involvement in tracheal morphogenesis. We found that Vari is distributed in the cytoplasm of the optic lobe neuroepithelium, as well as in a subset of neuroblasts and differentiated neurons of the nervous system. We reduced vari function during the development of adult epithelia with a partial rescue, RNA interference and generation of genetically mosaic tissue. All three approaches demonstrate that vari is required for the patterning and morphogenesis of adult epithelial hairs and bristles.Varicose is involved in scaffold assembly at the SJ and has a role in patterning and morphogenesis of adult epithelia.
DOI	10.1186/1471-213X-8-99
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	BMC Dev. Biol.
Title	BMC Developmental Biology
Publication Year	2002
ISBN/ISSN	1471-213X
Data from Reference
Aberrations (2)
	Df(2L)Exel7079 Df(2L)vari^48EP
Alleles (20)
	cora^k08713 dlg1⁷ Nrg^l4 Nrx-IV⁴³⁰⁴ Pomp^48EP Pomp^EY06518 Scer\GAL4^da.G32 Scer\GAL4^dpp.PU Scer\GAL4^sim.P3.7 vari^34P vari^48EP vari^03953b vari^B4 vari^B5 vari^GD13798 vari^GE13049 vari^K4 vari^L4 vari^Scer\UAS.cMa w^+mC
Constructs (7)
	P{?EP} P{dpp-GAL4.U} P{EP} P{GAL4-da.G32} P{GAL4-sim.3.7} P{GD13798} P{UAS-vari.M}
Genes (15)
	Atpα cora crb dlg1 elav Fas3 Nrg Nrx-IV Pomp pros repo Scer\GAL4 vari w α-Spec
Insertions (4)
	P{?EP}vari^48EP P{?EP}vari^B4 P{?EP}vari^B5 P{EP}vari^GE13049
Natural transposons (1)
	P-element