Reference Report

Reference
Citation	Xu, X.L., Li, Y., Wang, F., Gao, F.B. (2008). The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila. J. Neurosci. 28(46): 11883--11889. (Export to RIS)
FlyBase ID	FBrf0206327
Publication Type	Research paper
PubMed ID	19005053
PubMed Abstract	Fragile X syndrome is the most common form of inherited mental retardation caused by loss of the fragile X mental retardation protein 1 (FMRP). The detailed molecular pathways underlying the pathogenesis of this disorder remain incompletely understood. Here, we show that miR-124a, a nervous-system-specific miRNA, is associated with the Drosophila homolog of FMRP (dFMR1) in vivo. Ectopic expression of wild-type but not mutant miR-124a precursors decreased dendritic branching of dendritic arborization sensory neurons, which was partially rescued by the loss of dFMR1 activity, suggesting that the biogenesis and/or function of miR-124a are partially dependent on dFMR1. Indeed, in contrast with the complete loss of mature miR-124a in Dicer-1 mutants, steady-state levels of endogenous or ectopically expressed mature miR-124a were partially reduced in dfmr1 mutants, whereas the level of pre-miR-124a increased. This effect could be explained in part by the reduced abundance of the Dicer-1-Ago1 complex in the absence of dFMR1. These findings suggest a modulatory role for dFMR1 to maintain proper levels of miRNAs during neuronal development.
DOI	10.1523/JNEUROSCI.4114-08.2008
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (13)
	Avic\GFP^Scer\UAS.cUa Dcr-1^Q1147X Dcr-1^Q1948X Fmr1³ Fmr1^4.L mir-9a^Scer\UAS.cLa mir-124^mut.Scer\UAS mir-124^Scer\UAS.cXa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^109(2)80 Scer\GAL4²²¹ Scer\GAL4⁴⁷⁷ Scer\GAL4^mir-124.PX
Constructs (5)
	P{mir-124-GAL4.X} P{UAS-GFP.U} P{UAS-mir-9a.L} P{UAS-pre-mir-124a.mut} P{UAS-pre-mir-124a}
Genes (7)
	AGO1 Avic\GFP Dcr-1 Fmr1 mir-9a mir-124 Scer\GAL4
Insertions (3)
	P{GawB}109(2)80 P{GawB}221 P{GawB}477
Natural transposons (1)
	P-element
Transcripts (3)
	Scer\GAL4²²¹RA Scer\GAL4⁴⁷⁷RA Scer\GAL4^mir-124.PXRA