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Citation
Sural, T.H., Peng, S., Li, B., Workman, J.L., Park, P.J., Kuroda, M.I. (2008). The MSL3 chromodomain directs a key targeting step for dosage compensation of the Drosophila melanogaster X chromosome.  Nat. Struct. Mol. Biol. 15(12): 1318--1325.
FlyBase ID
FBrf0206341
Publication Type
Research paper
Abstract
The male-specific lethal (MSL) complex upregulates the single male X chromosome to achieve dosage compensation in Drosophila melanogaster. We have proposed that MSL recognition of specific entry sites on the X is followed by local targeting of active genes marked by histone H3 trimethylation (H3K36me3). Here we analyze the role of the MSL3 chromodomain in the second targeting step. Using ChIP-chip analysis, we find that MSL3 chromodomain mutants retain binding to chromatin entry sites but show a clear disruption in the full pattern of MSL targeting in vivo, consistent with a loss of spreading. Furthermore, when compared to wild type, chromodomain mutants lack preferential affinity for nucleosomes containing H3K36me3 in vitro. Our results support a model in which activating complexes, similarly to their silencing counterparts, use the nucleosomal binding specificity of their respective chromodomains to spread from initiation sites to flanking chromatin.
PubMed ID
PubMed Central ID
PMC2636508 (PMC) (EuropePMC)
Related Publication(s)
Note
Chromodomain-mediated spreading on active genes.
Hosey and Brand, 2009, Nat. Struct. Mol. Biol. 16(1): 11--13 [FBrf0215331]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Struct. Mol. Biol.
    Title
    Nature Structural and Molecular Biology
    Publication Year
    2004-
    ISBN/ISSN
    1545-9993 1545-9985
    Data From Reference
    Alleles (7)
    Genes (7)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (6)