Reference Report

Reference
Citation	Sural, T.H., Peng, S., Li, B., Workman, J.L., Park, P.J., Kuroda, M.I. (2008). The MSL3 chromodomain directs a key targeting step for dosage compensation of the Drosophila melanogaster X chromosome. Nat. Struct. Mol. Biol. 15(12): 1318--1325. (Export to RIS)
FlyBase ID	FBrf0206341
Publication Type	Research paper
PubMed ID	19029895
PubMed Abstract	The male-specific lethal (MSL) complex upregulates the single male X chromosome to achieve dosage compensation in Drosophila melanogaster. We have proposed that MSL recognition of specific entry sites on the X is followed by local targeting of active genes marked by histone H3 trimethylation (H3K36me3). Here we analyze the role of the MSL3 chromodomain in the second targeting step. Using ChIP-chip analysis, we find that MSL3 chromodomain mutants retain binding to chromatin entry sites but show a clear disruption in the full pattern of MSL targeting in vivo, consistent with a loss of spreading. Furthermore, when compared to wild type, chromodomain mutants lack preferential affinity for nucleosomes containing H3K36me3 in vitro. Our results support a model in which activating complexes, similarly to their silencing counterparts, use the nucleosomal binding specificity of their respective chromodomains to spread from initiation sites to flanking chromatin.
DOI	10.1038/nsmb.1520
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Struct. Mol. Biol.
Title	Nature Structural and Molecular Biology
Publication Year	2004-
ISBN/ISSN	1545-9993 1545-9985
Data from Reference
Alleles (7)
	msl-3¹ msl-3^{+t.T:Zzzz\TAP} msl-3^{LYT30A.T:Zzzz\TAP} msl-3^{SYD62A.T:Zzzz\TAP} msl-3^{W59G.T:Zzzz\TAP} msl-3^{ΔCD.T:Zzzz\TAP} roX2^t4.0
Constructs (6)
	M{msl-3-TAP.LYT30A} M{msl-3-TAP.SYD62A} M{msl-3-TAP.W59G} M{msl-3-TAP.ΔCD} M{msl-3-TAP} P{GMroX2}
Genes (8)
	His3 mof msl-1 msl-2 msl-3 roX1 roX2 T:Zzzz\TAP
Natural transposons (1)
	Dmau\mariner