Reference Report

Reference
Citation	Lin, C.H., Li, B., Swanson, S., Zhang, Y., Florens, L., Washburn, M.P., Abmayr, S.M., Workman, J.L. (2008). Heterochromatin Protein 1a Stimulates Histone H3 Lysine 36 Demethylation by the Drosophila KDM4A Demethylase. Mol. Cell 32(5): 696--706. (Export to RIS)
FlyBase ID	FBrf0206348
Publication Type	Research paper
PubMed ID	19061644
PubMed Abstract	Recent discoveries of histone demethylases demonstrate that histone methylation is reversible. However, mechanisms governing the targeting and regulation of histone demethylation remain elusive. Here we report that a Drosophila melanogaster JmjC domain-containing protein, dKDM4A, is a histone H3K36 demethylase. dKDM4A specifically demethylates H3K36me2 and H3K36me3 both in vitro and in vivo. Affinity purification and mass spectrometry analysis revealed that heterochromatin protein 1a (HP1a) associates with dKDMA4A. We found that the chromo shadow domain of HP1a and a HP1-interacting motif of dKDM4A are responsible for this interaction. HP1a stimulates the histone H3K36 demethylation activity of dKDM4A, and this stimulation depends on the H3K9me-binding motif of HP1a. Finally, we provide in vivo evidence suggesting that HP1a and dKDM4A interact with each other and that loss of HP1a leads to an increased level of histone H3K36me3. Collectively, these results suggest a function of HP1a in transcription facilitating H3K36 demethylation at transcribed and/or heterochromatin regions.
DOI	10.1016/j.molcel.2008.11.008
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell
Title	Molecular Cell
Publication Year	1997-
ISBN/ISSN	1097-2765 1097-4164
Data from Reference
Alleles (7)
	Kdm4A^KG04636 Kdm4A^{Scer\UAS.T:Ivir\HA1,T:Zzzz\FLAG} Kdm4A^{V423A.Scer\UAS.T:Ivir\HA1,T:Zzzz\FLAG} Scer\GAL4^Sgs3.PD Su(var)205⁴ Su(var)205⁵ w^+mC
Constructs (3)
	P{Sgs3-GAL4.PD} P{UAS-Kdm4A.HA.FLAG} P{UAS-Kdm4A.V423A.HA.FLAG}
Genes (10)
	His3 HP1b HP1c Kdm4A Kdm4B Scer\GAL4 Su(var)205 T:Ivir\HA1 T:Zzzz\FLAG w
Insertions (1)
	P{SUPor-P}Kdm4A^KG04636
Natural transposons (1)
	P-element