Reference Report

Reference
Citation	Caussinus, E., Colombelli, J., Affolter, M. (2008). Tip-Cell Migration Controls Stalk-Cell Intercalation during Drosophila Tracheal Tube Elongation. Curr. Biol. 18(22): 1727--1734. (Export to RIS)
FlyBase ID	FBrf0206360
Publication Type	Research paper
PubMed ID	19026547
PubMed Abstract	Branching morphogenesis remodels epithelial tissues into tubular networks. This process is crucial to many organs, from the insect trachea to the vertebrate vasculature. Although Drosophila tracheal development has been well characterized morphologically and genetically, very little is known about the forces involved during morphogenesis. The repertoire of cell behaviors underlying tracheal primary branch remodeling is limited to cell migration, cell-shape changes, and stalk-cell intercalation (SCI), a process in which cells insert in between cells previously in contact with each other.Here, we identify the major forces that contribute to tracheal primary branch remodeling by using genetic and microsurgery experiments. As the tip cells migrate, they elongate the branches and create a tensile stress. This tensile stress triggers SCI, which, in turn, allows the branches to further elongate.The mechanism that we describe contrasts with "convergent extension by cell intercalation" acting during Drosophila germ band extension (GBE), where cell intercalation is the cause of epithelium elongation. Surprisingly, in tracheal branches, one or two leading cells produce enough mechanical power to intercalate many lagging cells. This may apply to other tubular networks.
DOI	10.1016/j.cub.2008.10.062
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (17)
	Act5C^{Scer\UAS.T:Avic\GFP} bsk² CycA^C8LR1 dap⁴ Disc\RFP^{mCherry.Scer\UAS.T:Uuuu\nls} His2Av^{T:Disc\RFP-mRFP} ik2^GS11377 Moe^{btl.T:Disc\RFP-mRFP} salm^Scer\UAS.cKa Scer\GAL4^{btl.B123.Scer\FRT} Scer\GAL4^btl.B123 Scer\GAL4^btl.PS Scer\GAL4^en-e16E sqh^{RLC.T:Avic\GFP-S65T} sqh^{Scer\UAS.T:Avic\GFP} twi¹ α-Cat^{Scer\UAS.T:Avic\GFP-sg}
Constructs (11)
	P{btl(FRT.y⁺)GAL4} P{btl(-FRT)GAL4} P{btl-moe.mRFP1} P{GAL4-btl.S} P{His2Av-mRFP1} P{sqh-GFP.RLC} P{UAS-Act5C.T:GFP} P{UAS-mCherry.NLS} P{UAS-salm.K} P{UAS-sqh.GFP} P{UAS-α-Cat.T:GFP.sg}
Genes (15)
	Act5C bsk CycA dap Disc\RFP His2Av ik2 Moe salm Scer\GAL4 sqh T:Avic\GFP T:Uuuu\nls6 twi α-Cat
Insertions (1)
	P{en2.4-GAL4}e16E
Natural transposons (1)
	P-element