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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2008.12.28). Isolation and characterization of Df(2R)BSC268. (Export to RIS) | ||
| FlyBase ID | FBrf0206404 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
From: kcook@XXXXXXXXXXXXXXX
Subject: Isolation and characterization of Df(2R)BSC268 Date: December 28, 2008 1:15:49 PM GMT-05:00 Isolation and characterization of Df(2R)BSC268 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC268 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f00088 and P{XP}d09411. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f00088/P{XP}d09411 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC268 from the segment of PBac{WH}f00088 to the left of its FRT site and the segment of P{XP}d09411 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d09411 to be Release 3 genomic coordinate 4091649 on chromosome arm 2R. The Gene Disruption Project determined the insertion site of P{XP}d09411 to be Release 3 genomic coordinate 4091792 on arm 2R. This corresponds to 45A1 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f00088 on the Release 5 map is 44E2. Consequently, the cytological breakpoints of Df(2R)BSC268 are predicted to be 44E2;45A1. It failed to complement cmp44E1 and Rya-r44Fk04913. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (2)
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| Constructs (2)
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| Genes (2)
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| Insertions (3)
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