Isolation and characterization of Df(2R)BSC660
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC660 was isolated as a FLP recombinase-induced recombination event involving P{XP}d05179 and PBac{WH}CG5602f00902. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d05179/PBac{WH}CG5602f00902 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC660 from the segment of P{XP}d05179 to the left of its FRT site and the segment of PBac{WH}CG5602f00902 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC660 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 59F6;60A8. Df(2R)BSC660 failed to complement bgcn1, ken1 and eIF6k13214.