Reference Report

Reference
Citation	Christensen, S., Cook, K., Cook, K. (2008.12.28). Isolation and characterization of Df(3R)BSC678. (Export to RIS)
FlyBase ID	FBrf0206426
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	From: kcook@XXXXXXXXXXXXXXX Subject: Isolation and characterization of Df(3R)BSC678 Date: December 28, 2008 1:15:51 PM GMT-05:00 Isolation and characterization of Df(3R)BSC678 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC678 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}lbl^f02857 and P{XP}d07598. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w¹¹¹⁸; P{hs-hid}3, Dr¹/TM6C, Sb¹ cu¹ females crossed to P{hsFLP}1, y¹ w¹¹¹⁸; PBac{WH}lbl^f02857/P{XP}d07598 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC678 from the segment of PBac{WH}lbl^f02857 to the left of its FRT site and the segment of P{XP}d07598 to the right of its FRT site. Exelixis, Inc. determined the insertion site of PBac{WH}lbl^f02857 to be Release 3 genomic coordinate 17235248 on chromosome arm 3R. This corresponds to 93E1 on the Release 3 and Release 5 genome maps. The predicted position of P{XP}d07598 on the Release 5 map is 93F14. Consequently, the cytological breakpoints of Df(3R)BSC678 are predicted to be 93E1;93F14. Df(3R)BSC678 failed to complement tsl⁰⁰⁶¹⁷ and InR⁰⁵⁵⁴⁵. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX
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Language of Publication	English
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Data from Reference
Aberrations (1)
	Df(3R)BSC678
Alleles (2)
	InR⁰⁵⁵⁴⁵ tsl⁰⁰⁶¹⁷
Constructs (1)
	P+PBac{XP3.WH3}
Genes (2)
	InR tsl
Insertions (3)
	P{XP}Eip93F^d07598 P+PBac{XP3.WH3}BSC678 PBac{WH}lbl^f02857