Reference Report

Reference
Citation	Christensen, S., Cook, K., Cook, K. (2008.12.28). Isolation and characterization of Df(2R)BSC698. (Export to RIS)
FlyBase ID	FBrf0206445
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	From: kcook@XXXXXXXXXXXXXXX Subject: Isolation and characterization of Df(2R)BSC698 Date: December 28, 2008 1:19:53 PM GMT-05:00 Isolation and characterization of Df(2R)BSC698 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC698 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f00012 and P{XP}CG9812^d10315. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y¹ w¹¹¹⁸; PBac{WH}f00012/P{XP}CG9812^d10315 males crossed to w¹¹¹⁸; P{hs-hid}2, wg^Sp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC698 from the segment of PBac{WH}f00012 to the left of its FRT site and the segment of P{XP}CG9812^d10315 to the right of its FRT site. The cytological breakpoints of Df(2R)BSC698 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 59C1;59D11. Df(2R)BSC698 failed to complement twi¹ and l(2)06496⁰⁶⁴⁹⁶. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX
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Language of Publication	English
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Data from Reference
Aberrations (1)
	Df(2R)BSC698
Alleles (2)
	l(2)06496⁰⁶⁴⁹⁶ twi¹
Constructs (1)
	P+PBac{XP3.WH3}
Genes (2)
	l(2)06496 twi
Insertions (3)
	P{XP}CG9812^d10315 P+PBac{XP3.WH3}BSC698 PBac{WH}f00012