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Christensen, S., Cook, K., Cook, K. (2008.12.28). Isolation and characterization of Df(2R)BSC703. 
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FBrf0206454
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From: kcook@XXXX
Subject: Isolation and characterization of Df(2R)BSC703
Date: December 28, 2008  1:20:25  PM GMT- 05:00 
Isolation and characterization of Df(2R)BSC703
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC703 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}e04660 and P{XP}d01004. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; PBac{RB}e04660/P{XP}d01004 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC703 from the segment of PBac{RB}e04660 to the left of its FRT site and the segment of P{XP}d01004 to the right of its FRT site. The cytological breakpoints of Df(2R)BSC703 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 47F1;47F6. Df(2R)BSC703 failed to complement ix1.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    English
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)