Reference Report

Reference
Citation	Huang, J., Zhou, W., Watson, A.M., Jan, Y.N., Hong, Y. (2008). Efficient ends-out gene targeting in Drosophila. Genetics 180(1): 703--707. (Export to RIS)
FlyBase ID	FBrf0206486
Publication Type	Research paper
PubMed ID	18757917
PubMed Abstract	In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.
DOI	10.1534/genetics.108.090563
Related Publication(s)
Supplementary material	Supplemental Figures and Material. [FBrf0206395]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (7)
	crb^{mEosFP.KI.Scer\SceI.RS} rpr^Scer\UAS.cHa Scer\GAL4^221w- Scer\GAL4^477w- w^+* w^GMR.PH W^hs.PG
Constructs (5)
	P{FRT(Arf51F.KO)} P{FRT(crb::mEosFP.KI)} P{hs-hid} pRK1 pRK2
Genes (6)
	Arf51F crb rpr Scer\GAL4 W w
Insertions (2)
	P{?GawB}221w- P{?GawB}477w-
Natural transposons (1)
	P-element