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Cook, K. (2008.12.4). Extinct Exelixis Deletions. 
FlyBase ID
FBrf0206661
Publication Type
Personal communication to FlyBase
Abstract
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PubMed Central ID
Text of Personal Communication
Stocks of most of the deletions described in Parks et al. (2004). "Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome."  Nature Genetics 36: 288-292 ( http://flybase.org/reports/FBrf0175003.html) were transferred from Exelixis, Inc. to the Bloomington Drosophila Stock Center for public distribution. Some deletions from this publication, however, do not exist in stock. They were either lost prior to transfer, the stocks did not carry the described deletion or the stocks were discarded due to other problems. Some chromosomes identified as deletions by PCR tests may have carried other FLP-induced, non-deletion rearrangements, but the nature of these "false positives" is not known. The scientists at Exelixis, Inc. did not have time to characterize the deletion strains fully before releasing them to the public, so we undertook quality control complementation tests in Bloomington to cull the stocks that did not carry the described deletion.
The following list gives deletions that no longer exist. The rationale for discarding a stock is noted. The phenotypes scored in complementation tests are abbreviated as l=lethality, fs=female sterility, ms=male sterility and v=visible phenotype.
Df(1)Exel6222
Homozygotes present in stock
Df(1)Exel6224
Complemented Df(1)su(s)83 (l, fs)
Df(1)Exel6228
Homozygotes present in stock
Complemented dor1 (v)
Df(1)Exel6246
Homozygotes present in stock
Df(1)Exel6247
Homozygotes present in stock
Complemented g1 (v)
Also analyzed by Reza Farahani who concluded the stock is no good.
Df(1)Exel6249
Homozygotes present in stock
Df(1)Exel6250
Homozygotes present in stock
Df(1)Exel9052
Breakpoints identical to Df(1)Exel9049, which was retained.
Df(2L)Exel6019
Homozygotes present in stock
Complemented Df(2L)XE-2750 (l, fs), Df(2L)BSC41 (l, fs)
Df(2L)Exel6020
Homozygotes present in stock
Complemented Su(var)2055 (l, fs), Btk29Ak00206 (l, fs), Btk29AEP2167 (l, fs)
Df(2L)Exel6026
Complemented bsk1 (l, fs), chico1 (l, fs), nmd08774 (l, fs), trk1 (l, fs)
Larry Madden also reported that this stock is no good: chico, bsk, pten, trk, nmd all complemented in his tests. Payal Ray showed that Df was not present by PCR tests.
Df(2L)Exel6037
John Roote complementation tested stock against mutations in the region and found no Df present.
Df(2L)Exel7009
Homozygotes present in stock
Complemented aop1 (l, fs), l(2)s5379s5379 (l, fs), Df(2L)Exel6007 (l, fs), Df(2L)Exel7010, (l, fs), Df(2L)Exel6006 ((l, fs))
Df(2L)Exel7017
Homozygotes present in stock
Complemented sobd06074 (l, fs), odd5 (l, fs), Df(2L)drm-P2 (l, fs), Df(2L)Exel7018 (l, fs)
Df(2L)Exel7061
Homozygotes present in stock
Complemented ck13  (v, l, fs), l(2)35Bb1 (l, fs), l(2)35Bd4 (l, fs), l(2)35Bg2 (l, fs), TfIIS2 (l, fs)
Df(2L)Exel7065
Homozygotes present in stock
John Roote complementation tested stock against mutations in the region and found no Df present in the stock.
Df(2L)Exel7082
Homozygotes present in stock
Complemented cul-202074 (l, fs), tsh04319 (l, fs), Df31k05815 (l, fs), Df(2L)Exel7081 (l, fs)
Df(2L)Exel8001
Complemented Df(2L)BSC4 (l, fs), U2af3806751 (l, fs), Df(2L)al (l, fs), Df(2L)net-PMF (l, fs), U2af38k14504 (l, fs)
Df(2L)Exel8004
Homozygotes present in stock
Laura Torroja showed Nlaz gene sequences were present by PCR.
Df(2L)Exel8011
Homozygotes present in stock
Complemented RpL27AKG01128 (l, fs), tutl01085 (l, fs), Df(2L)dp-h25 (l, fs), dpov1 (v)
Df(2L)Exel8021
Homozygotes present in stock
Should delete grk, so should have balancer.
Df(2L)Exel8024
Homozygotes present in stock
Should delete RpL7, but no Minute phenotype present.
Df(2L)Exel8029
Homozygotes present in stock
Complemented Df(2L)BSC31 (l, fs)
Jim Erikson found no Df present by PCR. 
Df(2L)Exel8032
Homozygotes present in stock
Complemented Df(2L)fn7 (l, fs), l(2)34Fak00811 (l, fs), wb09437 (l, fs), l(2)34Fa1 (l, fs), wbSF11 (l, fs)
Df(2L)Exel9023
Complemented Df(2L)ED778 (l, fs), Df(2L)ED784 (l, fs), Df(2L)Exel7055 (l, fs), Df(2L)Exel8029 (l, fs)
Df(2L)Exel9027
Complemented bur1 (l, fs), Df(2L)Exel6047 (l, fs), Acon07054 (l, fs)
Df(2L)Exel9063
Breakpoints identical to Df(2L)Exel9033, which was retained.
Df(2R)Exel6073
Complemented Pu2 (l, fs), Df(2R)PL3 (l, fs), Df(2R)Exel6072 (l, fs)
Df(2R)Exel6081
Homozygotes present in stock
Complemented eIF-5A01296 (l, fs), enok1 (l, fs), gek09373 (l, fs), tsrk05633 (l, fs)
Df(2R)Exel6281
Homozygotes present in stock
Complemented bs1 (v), slbo01310 (fs)
Df(2R)Exel7112
Homozygotes present in stock
Complemented Df(2R)stan2 (l, fs), Df(2R)E3363 (l, fs)
Df(2R)Exel7129
Homozygotes present in stock
Complemented mam8 (l, fs), mam2 (l, fs), Cp1llcnbw38  (l, fs), AGO104845 (l, fs), Df(2R)50C-129 (l, fs)
Df(2R)Exel7133
Complemented Df(2R)03072 (l, fs), Df(2R)Exel6284 (l, fs)
Df(2R)Exel7139
Complemented Df(2R)Jp4 (l), Df(2R)Jp1 (l)
Df(2R)Exel7147
Complemented cnkE-2083 (l, fs), cnkk16314 (l, fs), Df(2R)BSC44 (l, fs), Df(2R)Exel6066 (l, fs)
Daniel Barbash showed no Df present by PCR.
Df(2R)Exel7152
Homozygotes present in stock
Complemented Hsf2 (l, fs), stau1 (l, fs), Df(2R)PC4 (l, fs)
Df(2R)Exel7163
Complemented Df(2R)AA21 (l, fs), Df(2R)D4 (l, fs), Df(2R)BSC19 (l, fs), l(22)0551005510 (l, fs)
Df(2R)Exel7167
Homozygotes present in stock
Complemented Df(2R)AA21 (l, fs), Df(2R)F36 (l, fs), Df(2R)Exel6072 (l, fs), Df(2R)Exel6073 (l, fs), Xbp1k13803 (l, fs)
Df(2R)Exel7178
Mini-white present.
Df(2R)Exel7174
Homozygotes present in stock
Complemented Df(2R)X58-8 (l), Df(2R)X58-12 (l)
Df should delete two Minute loci, RpS16 and RpS24, but stock doesn't show a Minute phenotype.
Df(2R)Exel7176
Homozygotes present in stock
Complemented Df(2R)59AD (l, fs), Df(2R)3-70 (l, fs)
Judy Kassis showed that CG9895 could be PCR amplified from homozygotes. 
Df(2R)Exel7179
Homozygotes present in stock
Complemented chrwk06908 (l, fs), Df(2R)bw-HB132 (l, fs), Df(2R)bw5 (l, fs), bw1 (v)
Df(2R)Exel7184
This distal 2R Df was in a region balanced poorly by CyO.  The stock broke down and the Df could not be recovered.
Df(2R)Exel7185
This distal 2R Df was in a region balanced poorly by CyO.  The stock broke down and the Df could not be recovered.
Df(2R)Exel8048
Homozygotes present in stock
Complemented PginNC1 (l, fs), Df(2R)Np3 (l, fs), Df(2R)Np1 (l, fs), Dmnk16109 (l, fs)
Df(2R)Exel8049
Complemented Mmp2k00604 (l, fs), Mmp202353 (l, fs), Mmp2EY08942 (l, fs), Mmp2DG24601 (l, fs), Map60KG00506 (l, fs)
Df(2R)Exel9021
Complemented Df(2R)nap19 (l, fs), Df(2R)nap1 (l, fs)
Df(2R)Exel9022
Homozygotes present in stock
Df(2R)Exel9024
Complemented Phmk07623 (l, fs), Df(2R)Exel7182 (l, fs), Df(2R)Chig230 (l, fs)
Df(3L)Exel6100
Homozygotes present in stock
Complemented RfC40B6 (l, fs), RfC40A18 (l, fs), Df(3L)ED4341 (l, fs), masxs76 (l, fs), RopG27 (l, fs)
Df(3L)Exel6111
Complemented Cdc27L7123 (l, fs), NeosL2249 (l, fs), Df(3L)pbl-X1 (l, fs), Df(3L)Exel6110 (l)
Df(3L)Exel6113
Homozygotes present in stock
Complemented smg1  (l, fs), bol1 (l, fs, ms), Df(3L)ED4421 (l, fs), mRpL7-L1210534 (l, fs)
Df(3L)Exel6124
Complemented shd2 (l, fs), Df(3L)XG12 (l, fs), Df(3L)ED4543 (l, fs)
Df(3L)Exel6139
Homozygotes present in stock
Complemented Df(3L)ED231 (l, fs), Df(3L)ED5017 (l, fs)
Df(3L)Exel6261
Complemented Df(3L)BSC12 (l, fs), Syx1301470 (l, fs), In(3LR)C190LUbx42TR (l, fs), trnS064117 (l, fs), Df(3L)ED4502 (l, fs)
Df(3L)Exel9005
Df(3L)ED4685 (l, fs), Complemented Df(3L)ED4710 (l, fs), Eip74EFDL-1  (l, fs), Df(3L)Exel6132 (l, fs), PepKG00294 (l, fs)
Df(3R)Exel6175
Complemented Df(3R)Exel7326 (l, fs), Df(3R)Exel8162 (l, fs), Df(3R)Exel7327 (l, fs)
Df(3R)Exel6177
Homozygotes present in stock
Complemented osa2 (l, fs), Df(3R)ED5780 (l, fs)
Bill Gilliland tested stock by PCR and found no Df.
Df(3R)Exel6219
Complemented Df(3R)awd-KRB (l, fs), Df(3R)tll-e (l, fs)
Joan Hooper says this complements Grk2 and doesn't show expected genetic interactions.
Df(3R)Exel6220
Complemented Df(3R)awd-KRB (l, fs), ttk1 (l, fs), awdj2A4 (l, fs), l(3)s1921s1921 (l, fs), ttk1e11 (l, fs)
Df(3R)Exel6258
Homozygotes present in stock
Complemented Df(3R)p-XT103 (l, fs), neur11 (l, fs), neur1 (l, fs)
Df(3R)Exel6260
Homozygotes present in stock
Complemented dco2 (l, fs), l(3)0367003670 (l, fs), Df(3R)tll-e (l, fs), Df(3R)R133 (l)
Df(3R)Exel6266
Complemented hth5E04 (l, fs), hth05745 (l, fs), Df(3R)BSC38 (l, fs)
Df(3R)Exel6268
Homozygotes present in stock
Complemented Df(3R)sbd104 (l, fs), tara03881  (l, fs), Df(3R)Exel7329 (l, fs), tara1 (l, fs)
Df(3R)Exel6271
Homozygotes present in stock
Complemented Df(3R)H-B79 (l, fs), Df(3R)Dl-BX12 (l, fs)
Df(3R)Exel6286
Homozygotes present in stock
Complemented Df(3R)BSC24 (l, fs), Aats-trp03559 (l, fs), Df(3R)by10 (l, fs), Df(3R)by416 (l, fs), Df(3R)Exel9036 (l, fs)
Df(3R)Exel6287
Complemented sr1 (v)
Df(3R)Exel7323
Complemented Hsc70-403550 (l, fs), Su(var)3-91 (l, fs), Hsc70-454.1 (l, fs), Tm102299 (l, fs)
Failed to suppress position effect variegation of In(1)wm4h.
Df(3R)Exel8156
Complemented Df(3R)ry81 (l, fs), Df(3R)kar-Sz8 (l, fs)
Df(3R)Exel8160
Complemented effmer1 (l), eff8 (fs), effs1782 (l)
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