Reference Report

Reference
Citation	Chen, J., Zhang, Y., Shen, P. (2008). A protein kinase C activity localized to neuropeptide Y-like neurons mediates ethanol intoxication in Drosophila melanogaster. Neuroscience 156(1): 42--47. (Export to RIS)
FlyBase ID	FBrf0206821
Publication Type	Research paper
PubMed ID	18675322
PubMed Abstract	Neuropeptide Y (NPY) regulates acute ethanol sensitivity and voluntary alcohol consumption in rodents. In Drosophila melanogaster, NPY-like neuropeptide F (NPF) and its receptor NPFR1 display a parallel function, suggesting that an evolutionarily conserved mechanism may underlie similar behavioral effects of ethanol in diverse organisms. We have used the fly model to uncover novel genes and molecular pathways important for acute ethanol response. Here we report a critical role of the conserved protein kinase C (PKC) pathway in mediating the intoxicating effect of ethanol. Flies expressing a pseudo-substrate inhibitor of PKC, directed by npf-gal4, displayed decreased ethanol sensitivity. Furthermore, the RNA interference analysis suggests that a calcium-independent PKC isoform (PKC98E, related to mammalian novel PKCs) is largely responsible for the behavioral phenotype. Finally, we provide evidence that the NPF/PKC-dependent mechanism selectively affects acute sensitivity but not rapid tolerance to ethanol intoxication. These findings reveal an uncharacterized role of PKC in NPY/NPF-mediated acute ethanol sensitivity in flies and possibly mammals.
DOI	10.1016/j.neuroscience.2008.07.008
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuroscience
Title	Neuroscience
Publication Year	1976-
ISBN/ISSN	0306-4522
Data from Reference
Alleles (8)
	Dscam^{exon17.1.Scer\UAS.T:Avic\GFP} inaC^{PKCi.Scer\UAS} n-syb^{Scer\UAS.cEa.T:Avic\GFP} Pka-R1^BDK.Scer\UAS Pkc53E^GD11984 Pkc98E^VDRC.cUa Scer\GAL4^elav-C155 Scer\GAL4^npf.1
Constructs (7)
	P{GD11984} P{npf-GAL4.1} P{UAS-Dscam.exon17.1.GFP} P{UAS-n-syb-GFP.E} P{UAS-Pka-R1.BDK} P{UAS-PKCi.B} P{VDRC.Pkc98E.U}
Genes (8)
	Dscam inaC npf n-syb Pka-R1 Pkc53E Pkc98E Scer\GAL4
Insertions (1)
	P{GawB}elav^C155
Natural transposons (1)
	P-element