Reference Report

Reference
Citation	Kelley, R.L., Lee, O.K., Shim, Y.K. (2008). Transcription rate of noncoding roX1 RNA controls local spreading of the Drosophila MSL chromatin remodeling complex. Mech. Dev. 125(11-12): 1009--1019. (Export to RIS)
FlyBase ID	FBrf0206905
Publication Type	Research paper
PubMed ID	18793722
PubMed Abstract	The dosage compensation complex in Drosophila is composed of at least five MSL proteins and two noncoding roX RNAs that bind hundreds of sites along the single male X chromosome. The roX RNAs are transcribed from X-linked genes and their RNA products "paint" the male X. The roX RNAs and bound MSL proteins can spread in cis from sites of roX transcription, but the mechanism controlling spreading is unknown. Here we find that cis spreading from autosomal roX1 transgenes is coupled to the level of roX transcription. Low to moderate transcription favors, and vigorous transcription abolishes local spreading. We constructed a roX1 minigene one third the size of wild type as a starting point for mutagenesis. This allowed us to test which evolutionarily conserved motifs were required for activity. One short repeat element shared between roX1 and roX2 was found to be particularly important. When all copies were deleted, the RNA was inactive and unstable, while extra copies seem to promote local spreading of the MSL complex from sites of roX1 synthesis. We propose that assembly of the MSL proteins onto the extreme 3' region of elongating roX1 transcripts determines whether the MSL complex spreads in cis.
DOI	10.1016/j.mod.2008.08.003
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Aberrations (1)
	Df(1)52
Alleles (21)
	roX1::Dpse\roX1^{Δ39pseudo.Hsp83} roX1^ex6 roX1^Hsp83.PM roX1^MB710 roX1^Scer\UAS.cKa roX1^t4.9 roX1^Δ39 roX1^ΔAB.Hsp83 roX1^ΔIRB.Hsp83 roX1^{ΔRB1-3.Hsp83} roX1^ΔRB1.Hsp83 roX1^ΔRB2.Hsp83 roX1^ΔRB3.Hsp83 roX1^{ΔRB123.Hsp83} roX1^ΔReg1.Hsp83 roX1^ΔSL1.Hsp83 roX1^ΔSL2.Hsp83 Scer\GAL4^Act5C.PU Scer\GAL4^hs.PB Scer\GAL4^tub.PU Scer\GAL80^ts.αTub84B
Constructs (14)
	P{Act5C-GAL4.U} P{GAL4-Hsp70.PB} P{GMroX1} P{H83-roX1.ΔAB} P{H83-roX1.ΔIRB} P{H83-roX1.ΔReg1} P{H83-roX1.ΔSL1} P{H83-roX1.ΔSL2} P{H83-roX1-Dpse\roX1} P{Hsp83-roX1.Δ39} P{Hsp83-roX1} P{tub-GAL4.U} P{tubP-GAL80^ts} P{UAS-roX1.K}
Genes (6)
	msl-1 roX1 roX1::Dpse\roX1 roX2 Scer\GAL4 Scer\GAL80
Insertions (12)
	P{H83-roX1.ΔAB}VK11 P{H83-roX1.ΔIRB}VK11 P{H83-roX1.ΔRB1-3} P{H83-roX1.ΔRB1} P{H83-roX1.ΔRB2} P{H83-roX1.ΔRB3} P{H83-roX1.ΔRB123} P{H83-roX1.ΔReg1}VK11 P{H83-roX1.ΔSL1}VK11 P{H83-roX1.ΔSL2}VK11 P{Hsp83-roX1}87B P{UAS-roX1.K}29A
Natural transposons (1)
	P-element