Reference Report

Reference
Citation	Martin, A.C., Kaschube, M., Wieschaus, E.F. (2009). Pulsed contractions of an actin-myosin network drive apical constriction. Nature 457(7228): 495--499. (Export to RIS)
FlyBase ID	FBrf0206985
Publication Type	Research paper
PubMed ID	19029882
PubMed Abstract	Apical constriction facilitates epithelial sheet bending and invagination during morphogenesis. Apical constriction is conventionally thought to be driven by the continuous purse-string-like contraction of a circumferential actin and non-muscle myosin-II (myosin) belt underlying adherens junctions. However, it is unclear whether other force-generating mechanisms can drive this process. Here we show, with the use of real-time imaging and quantitative image analysis of Drosophila gastrulation, that the apical constriction of ventral furrow cells is pulsed. Repeated constrictions, which are asynchronous between neighbouring cells, are interrupted by pauses in which the constricted state of the cell apex is maintained. In contrast to the purse-string model, constriction pulses are powered by actin-myosin network contractions that occur at the medial apical cortex and pull discrete adherens junction sites inwards. The transcription factors Twist and Snail differentially regulate pulsed constriction. Expression of snail initiates actin-myosin network contractions, whereas expression of twist stabilizes the constricted state of the cell apex. Our results suggest a new model for apical constriction in which a cortical actin-myosin cytoskeleton functions as a developmentally controlled subcellular ratchet to reduce apical area incrementally.
DOI	10.1038/nature07522
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Alleles (12)
	Avic\GFP^gish-Spider Moe^{hs.T:Avic\GFP} shg^R69 shg^{Ubi-p63E.T:Avic\GFP-rs} sna¹⁸ sna^twi.c.PE sqh^AX3 sqh^{RLC.T:Avic\GFP-S65T} sqh^{T:Disc\RFP-mCherry} T:Disc\RFP^mCherry twi^ey53 zip^{FL.Scer\UAS.T:Avic\GFP}
Constructs (6)
	P{hs-GFP-Moe} P{Psna_c} P{sqh-GFP.RLC} P{sqh-mCherry.M} P{UAS-zip.GFP.FL} P{Ubi-p63E-shg.GFP}
Genes (8)
	Avic\GFP gish Moe shg sna sqh twi zip
Insertions (1)
	P{PTT-GB}gish^Spider