Reference Report

Reference
Citation	Mitchell, N., Cranna, N., Richardson, H., Quinn, L. (2008). The Ecdysone-inducible zinc-finger transcription factor Crol regulates Wg transcription and cell cycle progression in Drosophila. Development 135(16): 2707--2716. (Export to RIS)
FlyBase ID	FBrf0207008
Publication Type	Research paper
PubMed ID	18614577
PubMed Abstract	The steroid hormone Ecdysone is crucial for developmental cell death, proliferation and morphogenesis in Drosophila. Herein, we delineate a molecular pathway linking Ecdysone signalling to cell cycle regulation in the Drosophila developing wing. We present evidence that the Ecdysone-inducible zinc-finger transcription factor Crol provides a crucial link between the Ecdysone steroid hormone pathway and the Wingless (Wg) signalling pathway in Drosophila. We identified Crol as a strong enhancer of a wing phenotype generated by overexpression of the Wg-inducible cell cycle inhibitor Hfp. We demonstrate that Crol is required for cell cycle progression: crol mutant clones have reduced cell cycles and are removed by apoptosis, while upregulation of Crol overrides the Wg-mediated developmental cell cycle arrest in the zone of non-proliferating cells in the wing disc. Furthermore, we show that Crol acts to repress wg transcription. We also show that overexpression of crol results in downregulation of Hfp, consistent with the identification of the crol mutant as a dominant enhancer of the Hfp overexpression phenotype. Taken together, our studies have revealed a novel mechanism for cell cycle regulation, whereby Crol links steroid hormone signals to Wg signalling and the regulation of crucial cell cycle targets.
DOI	10.1242/dev.021766
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (24)
	arm⁴ Avic\GFP^mus209.PT Avic\GFP^Scer\UAS.cUa Axn^E77 BacA\p35^Scer\UAS.cHa crol^k05205 crol^Scer\UAS.cMa CycB² CycE^AR95 dm^P0 E2f⁹¹ Ecol\lacZ^dm-PL35 Ecol\lacZ^stg.β-E6.4 Ecol\lacZ^wg-en11 EcR^{A-ΔC655.F645A.Scer\UAS} pUf68^Scer\UAS.cQa Scer\GAL4^Act5C.PP Scer\GAL4^Act.PU Scer\GAL4^en-e16E Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL4^tub.PU sgg¹ stg^AR2
Constructs (10)
	P{Act-GAL4.U} P{GAL4-Act5C(FRT.CD2).P} P{mus209-GFP} P{stg-lacZ.β-E6.4} P{tub-GAL4.U} P{UAS-crol.M} P{UAS-EcR.A.F645A} P{UAS-GFP.U} P{UAS-p35.H} P{UAS-pUf68.Q}
Genes (21)
	arm Avic\GFP Axn BacA\p35 ci crol CycB CycE dm E2f E(spl)m7-HLH Ecol\lacZ EcR en His3 mus209 pUf68 Scer\GAL4 sgg stg wg
Insertions (4)
	P{en1}wg^en11 P{en2.4-GAL4}e16E P{GawB}ptc^559.1 P{lacW}dm^PL35