Reference Report

Reference
Citation	Satoh, D., Sato, D., Tsuyama, T., Saito, M., Ohkura, H., Rolls, M.M., Ishikawa, F., Uemura, T. (2008). Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes. Nat. Cell Biol. 10(10): 1164--1171. (Export to RIS)
FlyBase ID	FBrf0207048
Publication Type	Research paper
PubMed ID	18758452
PubMed Abstract	Dendrites allow neurons to integrate sensory or synaptic inputs, and the spatial disposition and local density of branches within the dendritic arbor limit the number and type of inputs. Drosophila melanogaster dendritic arborization (da) neurons provide a model system to study the genetic programs underlying such geometry in vivo. Here we report that mutations of motor-protein genes, including a dynein subunit gene (dlic) and kinesin heavy chain (khc), caused not only downsizing of the overall arbor, but also a marked shift of branching activity to the proximal area within the arbor. This phenotype was suppressed when dominant-negative Rab5 was expressed in the mutant neurons, which deposited early endosomes in the cell body. We also showed that 1) in dendritic branches of the wild-type neurons, Rab5-containing early endosomes were dynamically transported and 2) when Rab5 function alone was abrogated, terminal branches were almost totally deleted. These results reveal an important link between microtubule motors and endosomes in dendrite morphogenesis.
DOI	10.1038/ncb1776
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Aberrations (1)
	Df(1)JA21
Alleles (21)
	Avic\GFP^{S65T.Scer\UAS} Avic\GFP^{Scer\UAS.T:Hsap\MYC,T:Mmus\2xFYVE} Avic\GFP^{Venus.Scer\UAS.T:Hsap\pm} Disc\RFP^{Scer\UAS.T:Myr-Src64B} Dlic¹ Dlic^{Scer\UAS.cSa.T:Avic\GFP-EGFP} Eb1^{Scer\UAS.T:Avic\GFP-EGFP} Khc²⁷ Lis-1^G10.14 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rab5² Rab5^{N142I.Scer\UAS} Rab5^{S43N.Scer\UAS} Rab5^{Scer\UAS.T:Avic\GFP} robl^MB Scer\GAL4^elav.PLu Scer\GAL4^Kr.PM Scer\GAL4^NP1015 Scer\GAL4^ppk.1.9 Scer\GAL4^unspecified T:Avic\GFP^EGFP
Constructs (14)
	P{GAL4} P{GAL4-elav.L} P{GAL4-Kr.C} P{ppk-GAL4.1.9} P{UAS-Dlic.GFP.S} P{UAS-Eb1.GFP} P{UAS-GFP.S65T} P{UAS-GFP-myc-2xFYVE} P{UAS-mCD8::GFP.L} P{UAS-myr-mRFP} P{UAS-Rab5.GFP} P{UAS-Rab5.S43N} P{UAS-Rab5^N142I} P{UAS-Venus.pm}
Genes (11)
	Avic\GFP Dhc64C Disc\RFP Dlic Eb1 Khc Lis-1 Mmus\Cd8a Rab5 robl Scer\GAL4
Insertions (1)
	P{GawB}Rip11^NP1015
Natural transposons (1)
	P-element