Reference Report

Reference
Citation	Lee, S., Zhou, L., Kim, J., Kalbfleisch, S., Schöck, F. (2008). Lasp anchors the Drosophila male stem cell niche and mediates spermatid individualization. Mech. Dev. 125(9-10): 768--776. (Export to RIS)
FlyBase ID	FBrf0207050
Publication Type	Research paper
PubMed ID	18655828
PubMed Abstract	Lasp family proteins contain an amino-terminal LIM domain, two actin-binding nebulin repeats and a carboxyl-terminal SH3 domain. Vertebrate Lasp-1 localizes to focal adhesions and the leading edge of migrating cells, and is required for cell migration. To assess the in vivo function of Lasp, we generated a null mutant in Drosophila Lasp. Lasp(1) is homozygous viable, but male sterile. In Lasp mutants the stem cell niche is no longer anchored to the apical tip of the testis, and actin cone migration is perturbed resulting in improper spermatid individualization. Hub cell mislocalization can by phenocopied by expressing Lasp or betaPS integrin RNAi transgenes in somatic cells, and Lasp genetically interacts with betaPS integrin, demonstrating that Lasp functions together with integrins in hub cells to anchor the stem cell niche. Finally, we show that the stem cell niche is maintained even if it is not properly localized.
DOI	10.1016/j.mod.2008.06.012
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Aberrations (2)
	Df(3L)Lasp¹ Df(3L)st7
Alleles (11)
	CG9692^MB03281 Lasp¹ Lasp^GD10498 Lasp^GD16308 Lasp^{Scer\UAS.T:Avic\GFP-EGFP} mys⁸ mys^GD15002 Scer\GAL4^C587 Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^ptc-559.1 T:Avic\GFP^EGFP
Constructs (5)
	P{GAL4::VP16-nos.UTR} P{GD10498} P{GD15002} P{GD16308} P{UAS-Lasp.GFP}
Genes (7)
	CG9692 Fas3 fax Lasp mys Scer\GAL4 vas
Insertions (5)
	Mi{ET1}Lasp^MB03281 P{GAL4}C587 P{GawB}ptc^559.1 PBac{RB}Lasp^e00056 PBac{RB}Lasp^e03191
Natural transposons (1)
	P-element