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| Citation | Rogers, G.C., Rusan, N.M., Peifer, M., Rogers, S.L. (2008). A multicomponent assembly pathway contributes to the formation of acentrosomal microtubule arrays in interphase Drosophila cells. Mol. Biol. Cell 19(7): 3163--3178. (Export to RIS) | ||
| FlyBase ID | FBrf0207058 | ||
| Publication Type | Research paper | ||
| PubMed ID | 18463166 | ||
| PubMed Abstract | In animal cells, centrosomes nucleate microtubules that form polarized arrays to organize the cytoplasm. Drosophila presents an interesting paradox however, as centrosome-deficient mutant animals develop into viable adults. To understand this discrepancy, we analyzed behaviors of centrosomes and microtubules in Drosophila cells, in culture and in vivo, using a combination of live-cell imaging, electron microscopy, and RNAi. The canonical model of the cycle of centrosome function in animal cells states that centrosomes act as microtubule-organizing centers throughout the cell cycle. Unexpectedly, we found that many Drosophila cell-types display an altered cycle, in which functional centrosomes are only present during cell division. On mitotic exit, centrosomes disassemble producing interphase cells containing centrioles that lack microtubule-nucleating activity. Furthermore, steady-state interphase microtubule levels are not changed by codepleting both gamma-tubulins. However, gamma-tubulin RNAi delays microtubule regrowth after depolymerization, suggesting that it may function partially redundantly with another pathway. Therefore, we examined additional microtubule nucleating factors and found that Mini-spindles, CLIP-190, EB1, or dynein RNAi also delayed microtubule regrowth; surprisingly, this was not further prolonged when we codepleted gamma-tubulins. Taken together, these results modify our view of the cycle of centrosome function and reveal a multi-component acentrosomal microtubule assembly pathway to establish interphase microtubule arrays in Drosophila. | ||
| DOI | 10.1091/mbc.E07-10-1069 | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Mol. Biol. Cell | ||
| Title | Molecular Biology of the Cell | ||
| Publication Year | 1992- | ||
| ISBN/ISSN | 1059-1524 | ||
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Data from Reference
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| Alleles (2)
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| Constructs (2)
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| Genes (19)
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| Natural transposons (1)
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