Reference Report

Reference
Citation	Carrera, I., Zavadil, J., Treisman, J.E. (2008). Two subunits specific to the PBAP chromatin remodeling complex have distinct and redundant functions during drosophila development. Mol. Cell. Biol. 28(17): 5238--5250. (Export to RIS)
FlyBase ID	FBrf0207089
Publication Type	Research paper
PubMed ID	18573871
PubMed Abstract	Chromatin remodeling complexes control the availability of DNA binding sites to transcriptional regulators. Two distinct conserved forms of the SWI/SNF class of complexes are characterized by the presence of specific accessory subunits. In Drosophila, the core Brahma complex associates either with Osa to form the BAP complex or with Bap170 and Bap180 to form the PBAP complex. osa mutations reproduce only a subset of the developmental phenotypes caused by mutations in subunits of the core complex. To test whether the PBAP complex performs the remaining functions, we generated mutations in bap170 and bap180. Surprisingly, we found that Bap180 is not essential for viability, although it is required in ovarian follicle cells for normal eggshell development. Bap170 is necessary to stabilize the Bap180 protein, but a mutant form that retains this function is sufficient for both survival and fertility. The two subunits act redundantly to allow metamorphosis; using gene expression profiling of bap170 bap180 double mutants, we found that the PBAP complex regulates genes involved in tissue remodeling and immune system function. Finally, we generated mutants lacking Bap170, Bap180, and Osa in the germ line to demonstrate that the core Brahma complex can function in oogenesis without any of these accessory subunits.
DOI	10.1128/MCB.00747-08
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (2)
	Df(2R)Bap170^Δ115 Df(2R)Bap170^Δ135
Alleles (19)
	Bap170^{dsRNA.Scer\UAS.WIZ} Bap170^Scer\UAS.cCa Bap170^Δ65 Bap170^Δ115 Bap170^Δ135 brm² Ecol\lacZ^Adh.EcRE osa^4H osa³⁰⁸ polybromo^EY14080 polybromo^Scer\UAS.cCa polybromo^Δ86 Scer\GAL4^ap-md544 Scer\GAL4^da.G32 Scer\GAL4^T155 Scer\GAL4^tub.PU Trap1^Δ115 Trap1^Δ135 w^+mC
Constructs (7)
	P{?EPgy2} P{EcRE.lacZ} P{GAL4-da.G32} P{tub-GAL4.U} P{UAS-Bap170.C} P{UAS-Bap170.WIZ} P{UAS-bap180.C}
Genes (92)
	ac Acer Arpc1 AttA AttB Bap170 Brd brm Cbl CG3823 CG5807 CG6429 CG7227 CG7290 CG8192 CG10477 CG11034 CG11319 CG11529 CG11865 CG12207 CG18563 CG31205 CG32302 Cht4 Cht6 ci Cpr65Ec Cpr67B Cpr67Fa2 CR33013 debcl dl Dl Drs Drsl5 E(spl)m4-BFM eater Ecol\lacZ EcR Eig71Eg elav Fadd fz GNBP3 grh He ImpE2 InR Jon25Biii Jon65Aii Jra Khc-73 Lcp65Ab2 LysX m mag Mbs Mmp1 msn MtnC Myo61F Myo95E nec obst-J osa pes PGRP-SD PhKγ Pka-C3 polybromo puc pug rhea RhoBTB Rhp rpr Scer\GAL4 srp Tab2 tok Tom Traf4 Trap1 Tsf3 w wg Zip3 zormin βTry εTry ηTry
Insertions (5)
	P{?EPgy2}Bap170^Δ135 P{EPgy2}polybromo^EY14080 P{EPgy2}Trap1^EY10238 P{GawB}ap^md544 P{GawB}T155
Natural transposons (1)
	P-element