Reference Report

Reference
Citation	Martin, R., Smibert, P., Yalcin, A., Tyler, D.M., Schäfer, U., Tuschl, T., Lai, E.C. (2009). A Drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways. Mol. Cell. Biol. 29(3): 861--870. (Export to RIS)
FlyBase ID	FBrf0207125
Publication Type	Research paper
PubMed ID	19047376
PubMed Abstract	Canonical primary microRNA (miRNA) transcripts and mirtrons are proposed to transit distinct nuclear pathways en route to generating mature approximately 22 nucleotide regulatory RNAs. We generated a null allele of Drosophila pasha, which encodes a double-stranded RNA-binding protein partner of the RNase III enzyme Drosha. Analysis of this mutant yielded stringent evidence that Pasha is essential for the biogenesis of canonical miRNAs but is dispensable for the processing and function of mirtron-derived regulatory RNAs. The pasha mutant also provided a unique tool to study the developmental requirements for Drosophila miRNAs. While pasha adult somatic clones are similar in many respects to those of dicer-1 clones, pasha mutant larvae revealed an unexpected requirement for the miRNA pathway in imaginal disc growth. These data suggest limitations to somatic clonal analysis of miRNA pathway components.
DOI	10.1128/MCB.01524-08
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (11)
	AGO2⁴¹⁴ Avic\GFP^{EGFP.mir-7.αTub84B} Avic\GFP^tub.mir-1004 Dcr-1^Q1147X Dcr-2^L811fsX loqs^KO mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} mir-1004^{Scer\UAS.T:Disc\RFP-DsRed2} pasha^+t4.1 pasha^KO w^+mC
Constructs (6)
	P{pasha^+t4.1} P{tub-EGFP.mir-7} P{tub-GFP-mir-1004} P{UAS-DsRed2-mir-7} P{UAS-DsRed2-mir-1004} PBac{RB3.WH3}
Genes (29)
	AGO2 Avic\GFP ban bft CG1792 Dcr-1 Dcr-2 elav loqs mir-1 mir-2a-1 mir-2a-2 mir-7 mir-8 mir-9a mir-10 mir-11 mir-14 mir-124 mir-276a mir-279 mir-305 mir-317 mir-1003 mir-1004 mir-1008 mir-1010 pasha w
Insertions (3)
	PBac{RB3.WH3}pasha^KO PBac{RB}e00907 PBac{WH}pasha^f07241
Natural transposons (1)
	P-element