Reference Report

Reference
Citation	Nakamura, R., Takeuchi, R., Takata, K., Shimanouchi, K., Abe, Y., Kanai, Y., Ruike, T., Ihara, A., Sakaguchi, K. (2008). TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster. Mol. Cell. Biol. 28(21): 6620--6631. (Export to RIS)
FlyBase ID	FBrf0207153
Publication Type	Research paper
PubMed ID	18765642
PubMed Abstract	The Saccharomyces cerevisiae poly(A) polymerases Trf4 and Trf5 are involved in an RNA quality control mechanism, where polyadenylated RNAs are degraded by the nuclear exosome. Although Trf4/5 homologue genes are distributed throughout multicellular organisms, their biological roles remain to be elucidated. We isolated here the two homologues of Trf4/5 in Drosophila melanogaster, named DmTRF4-1 and DmTRF4-2, and investigated their biological function. DmTRF4-1 displayed poly(A) polymerase activity in vitro, whereas DmTRF4-2 did not. Gene knockdown of DmTRF4-1 by RNA interference is lethal in flies, as is the case for the trf4 trf5 double mutants. In contrast, disruption of DmTRF4-2 results in viable flies. Cellular localization analysis suggested that DmTRF4-1 localizes in the nucleolus. Abnormal polyadenylation of snRNAs was observed in transgenic flies overexpressing DmTRF4-1 and was slightly increased by the suppression of DmRrp6, the 3'-5' exonuclease of the nuclear exosome. These results suggest that DmTRF4-1 and DmRrp6 are involved in the polyadenylation-mediated degradation of snRNAs in vivo.
DOI	10.1128/MCB.00448-08
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (10)
	Rrp6^f07001 Scer\GAL4^Act5C.PU Scer\GAL4^Bx-MS1096 Scer\GAL4^ey.PH Scer\GAL4^GMR.PF Scer\GAL4^hs.PB Trf4-1^{dsRNA.Scer\UAS.cNa} Trf4-1^Scer\UAS.cNa Trf4-2^NP2505 w^+mC
Constructs (6)
	P{Act5C-GAL4.U} P{GAL4-ey.H} P{GAL4-Hsp70.PB} P{GAL4-ninaE.GMR} P{UAS-Trf4-1.IR} P{UAS-Trf4-1.N}
Genes (41)
	CG3631 CG3731 CG7265 CG11120 CG11168 CG11771 CG14860 CG31220 CG33331 CG33332 CycC Fib Osbp Rrp6 Scer\GAL4 snRNA:U1:21D snRNA:U1:82Eb snRNA:U1:95Ca snRNA:U1:95Cb snRNA:U1:95Cc snRNA:U2:14B snRNA:U2:34ABa snRNA:U2:34ABb snRNA:U2:34ABc snRNA:U2:38ABa snRNA:U2:38ABb snRNA:U4:38AB snRNA:U4:39B snRNA:U5:14B snRNA:U5:23D snRNA:U5:34A snRNA:U5:35D snRNA:U5:38ABa snRNA:U5:38ABb snRNA:U5:63BC snRNA:U6:96Aa snRNA:U6:96Ab snRNA:U6:96Ac Trf4-1 Trf4-2 w
Insertions (3)
	P{GawB}Bx^MS1096 P{GawB}Trf4-2^NP2505 PBac{WH}Rrp6^f07001
Natural transposons (1)
	P-element